Online extraction and isolation of highly polar chemical constituents from Brassica napus L. pollen by high shear technique coupled with high-performance counter-current chromatography
文献类型：期刊论文
| 作者 | Feng ZF(冯祖飞)1,2,3; Chen XF(陈小芬)1,2,3; Di DL(邸多隆)1,2 ; Di DL(邸多隆)
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| 刊名 | Journal of Separation Science
 |
| 出版日期 | 2012 |
| 卷号 | 35期号:5-6页码:625-632 |
| ISSN号 | 1615-9306 |
| 关键词 | High shear technique (HSDE) Highly polar High-performance counter-current chromatography (HPCCC) Online extraction and isolation The pollen of Brassica napus L |
| 通讯作者 | 邸多隆 |
| 英文摘要 | High shear technique coupled with high-performance counter-current chromatography was successfully used for the extraction and online isolation of seven highly polar chemical constituents from the Brassica napusL. The lower phase of ethyl acetate–n-butanol–water (1:4:5, v:v:v) was used as both the high shear technique solvent and high-performance counter-current chromatography mobile phase. Seven compounds of 14.2 mg of uridine, 4.6 mg of xanthosine, 7.8 mg of guanosine, 5.3 mg of adenosine, 19.5 mg of kaempferol-3,4'-di-O-β-D-glucopyranoside, 17.7 mg of kaempferol-3-O-(2-O-β-D-glucopyranosy1)-β-D-glucopyranoside, and 25.7 mg of an unknown compound, with a high-performance liquid chromatography (HPLC) purity over 95.0%, were obtained in a one-step extraction-separation process within 130 min from 20.0 g of raw material of pollen of Brassica napusL. Moreover, the mode of elution–extrusion was employed for the separation of the last one compound. The isolated compounds were analyzed by HPLC, and the chemical structures of the compounds mentioned above were identified by UV and NMR. It is the first time to combine the high shear technique and high-performance counter-current chromatography for the online isolation of the nature products. |
| 学科主题 | 分析化学与药物化学 |
| 资助信息 | the "Hundred Talents Program" of Chinese Academy Sciences (CAS) in 2007;the Natiomal Natural Science Foundation of China (NSFC No. 21175142) |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000303040400002 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://210.77.64.217/handle/362003/19551]  |
| 专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| 通讯作者 | Di DL(邸多隆) |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Chem NW Plant Resources, Lanzhou, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng ZF,Chen XF,Di DL,et al. Online extraction and isolation of highly polar chemical constituents from Brassica napus L. pollen by high shear technique coupled with high-performance counter-current chromatography[J]. Journal of Separation Science,2012,35(5-6):625-632. |
| APA | Feng ZF,Chen XF,Di DL,&邸多隆.(2012).Online extraction and isolation of highly polar chemical constituents from Brassica napus L. pollen by high shear technique coupled with high-performance counter-current chromatography.Journal of Separation Science,35(5-6),625-632. |
| MLA | Feng ZF,et al."Online extraction and isolation of highly polar chemical constituents from Brassica napus L. pollen by high shear technique coupled with high-performance counter-current chromatography".Journal of Separation Science 35.5-6(2012):625-632. |
入库方式： OAI收割
来源：兰州化学物理研究所
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