Clostridium thermocellum Nitrilase Expression and Surface Display on Bacillus subtilis Spores
文献类型:期刊论文
作者 | Chen, Huayou1,2; Zhang, Tianxi1; Sun, Tengyun1; Ni, Zhong1; Le, Yilin1; Tian, Rui1; Chen, Zhi1; Zhang, Chunxia1 |
刊名 | JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY |
出版日期 | 2015 |
卷号 | 25期号:6页码:381-387 |
ISSN号 | 1464-1801 |
关键词 | Nitrilase Niacin Bacillus subtilis Clostridium thermocellum Spore surface 3-Cyanopyridine |
英文摘要 | Nitrilases are an important class of industrial enzymes. They require mild reaction conditions and are highly efficient and environmentally friendly, so they are used to catalyze the synthesis of carboxylic acid from nitrile, a process considered superior to conventional chemical syntheses. Nitrilases should be immobilized to overcome difficulties in recovery after the reaction and to stabilize the free enzyme. The nitrilase from Clostridium thermocellum was expressed, identified and displayed on the surface of Bacillus subtilis spores by using the spore coat protein G of B. subtilis as an anchoring motif. In a free state, the recombinant nitrilase catalyzed the conversion of 3-cyanopyridine to niacin and displayed maximum catalytic activity (8.22 units/mg protein) at 40 degrees C and pH 7.4. SDS-PAGE and Western blot were used to confirm nitrilase display. Compared with the free enzyme, the sporeimmobilized nitrilase showed a higher tolerance for adverse environmental conditions. After the reaction, recombinant spores were recovered via centrifugation and reused 3 times to catalyze the conversion of 3-cyanopyridine with 75.3% nitrilase activity. This study demonstrates an effective means of nitrilase immobilization via spore surface display, which can be applied in biological processes or conversion. (C) 2015 S. Karger AG, Basel |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
研究领域[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
关键词[WOS] | ENZYMES ; CLONING ; SYSTEM ; ACID |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000370260200003 |
源URL | [http://ir.ipe.ac.cn/handle/122111/20210] |
专题 | 过程工程研究所_管理及支撑部门 |
作者单位 | 1.Jiangsu Univ, Inst Life Sci, 301 Xuefu Rd, Zhenjiang, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China |
推荐引用方式 GB/T 7714 | Chen, Huayou,Zhang, Tianxi,Sun, Tengyun,et al. Clostridium thermocellum Nitrilase Expression and Surface Display on Bacillus subtilis Spores[J]. JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY,2015,25(6):381-387. |
APA | Chen, Huayou.,Zhang, Tianxi.,Sun, Tengyun.,Ni, Zhong.,Le, Yilin.,...&Zhang, Chunxia.(2015).Clostridium thermocellum Nitrilase Expression and Surface Display on Bacillus subtilis Spores.JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY,25(6),381-387. |
MLA | Chen, Huayou,et al."Clostridium thermocellum Nitrilase Expression and Surface Display on Bacillus subtilis Spores".JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY 25.6(2015):381-387. |
入库方式: OAI收割
来源:过程工程研究所
其他版本
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。