Directing membrane chromatography to manufacture alpha(1)-antitrypsin from human plasma fraction IV
文献类型：期刊论文
| 作者 | Fan, Jinxin1,2; Luo, Jianquan1; Song, Weijie1; Chen, Xiangrong1; Wan, Yinhua1 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2015-12-04 |
| 卷号 | 1423期号:DEC页码:63-70 |
| 关键词 | Membrane chromatography Plasma protein alpha(1)-Antitrypsin Ion-exchange Hydrophobic interaction Membrane adsorber |
| ISSN号 | 0021-9673 |
| 英文摘要 | The surging demand for plasma proteins, mainly driven by the growing market and the development of new therapeutic indications, is promoting manufacturers to improve the throughput of plasma proteins. Due to the inherent convective mass transfer, membrane chromatography has been proved to be an efficient approach for extracting a small amount of target proteins from large-volume feed. In this study, ai-antitrypsin (AAT) was extracted from human plasma fraction IV by a two-step membrane chromatography. An anion-exchange membrane chromatography (AEMC) was used to capture the plasma proteins in bind/elute mode, and the obtained effluent was further polished by a hydrophobic interaction membrane chromatography (HIMC) in flow-through mode. Under optimal conditions, the recovery and purity of MT achieved 87.0% and 0.58 MT/protein (g/g) by AEMC, respectively. After the precise polishing by HIMC, the purity of AAT was 1.22 MT/protein (g/g). The comparison results showed that membrane chromatography outperformed column chromatography in both steps because of its high throughput. This two-step membrane chromatography could obtain an MT recovery of 83.3% and an activity recovery of 91.4%. The outcome of this work not only offers an alternative process for protein purification from plasma, but also provides guidelines for manufacturing product from a large-volume feed with multi-components by membrane chromatography. (C) 2015 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | ION-EXCHANGE CHROMATOGRAPHY ; HYBRID BIOSEPARATION TECHNIQUE ; ALPHA(1) PROTEINASE-INHIBITOR ; SCALE ANTIBODY PURIFICATION ; MONOCLONAL-ANTIBODY ; ALPHA-1-ANTITRYPSIN ; CONCENTRATE ; WHEY ; STEP ; SEPARATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000366073000008 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/20143]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fan, Jinxin,Luo, Jianquan,Song, Weijie,et al. Directing membrane chromatography to manufacture alpha(1)-antitrypsin from human plasma fraction IV[J]. JOURNAL OF CHROMATOGRAPHY A,2015,1423(DEC):63-70. |
| APA | Fan, Jinxin,Luo, Jianquan,Song, Weijie,Chen, Xiangrong,&Wan, Yinhua.(2015).Directing membrane chromatography to manufacture alpha(1)-antitrypsin from human plasma fraction IV.JOURNAL OF CHROMATOGRAPHY A,1423(DEC),63-70. |
| MLA | Fan, Jinxin,et al."Directing membrane chromatography to manufacture alpha(1)-antitrypsin from human plasma fraction IV".JOURNAL OF CHROMATOGRAPHY A 1423.DEC(2015):63-70. |
入库方式： OAI收割
来源：过程工程研究所
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