Development of a thermally stable formulation for L-asparaginase storage in aqueous conditions
文献类型：期刊论文
| 作者 | Fu, Yankai1,2; Wu, Yanzhuo3; Wei, Yuping1; Chen, Xianhua3; Xu, Jiandong1; Xu, Xia1 |
| 刊名 | JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
 |
| 出版日期 | 2015-12-01 |
| 卷号 | 122期号:DEC页码:8-14 |
| 关键词 | L-Asparaginase Enzyme activity Denaturation Thermostable liquid formulation |
| ISSN号 | 1381-1177 |
| 英文摘要 | L-Asparaginase is an enzyme broadly used in pharmaceutical and food industry. Freeze-drying of L-asparaginase is time and energy consuming, and can cause enzyme activity loss. Furthermore, temperature excursion during transport and storage may ruin its activity as well. Hence, it is necessary to develop a thermostable liquid formulation of L-asparaginase. In this study, a thermostable liquid formulation using glycine as a protecting excipient was developed based on the screening tests. The presence of glycine in the citrate-phosphate buffer significantly increased the melting point by 4 degrees C and further dramatically slowed down the denaturation of L-asparaginase at 45 and 50 degrees C No subunit dissociation of L-asparaginase was detected by native-polyacrylamide gel electrophoresis, indicating that the denaturation of L-asparaginase was mainly caused by the changes in the secondary structure, confirmed by the circular dichroism data. This novel thermostable liquid formulation could offer significant promise for long-term storage of L-asparaginase in aqueous conditions. (C) 2015 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemistry & Molecular Biology ; Chemistry, Physical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | HIGHLY ACTIVATED SUPPORTS ; PROTEIN PHARMACEUTICALS ; MAILLARD REACTION ; ESCHERICHIA-COLI ; STABILITY ; ACRYLAMIDE ; THERMOSTABILITY ; INACTIVATION ; CAROTOVORA ; SUBSTRATE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000366078800002 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/20146]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Shuanglu Pharmaceut Co Ltd, Beijing 100043, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fu, Yankai,Wu, Yanzhuo,Wei, Yuping,et al. Development of a thermally stable formulation for L-asparaginase storage in aqueous conditions[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,2015,122(DEC):8-14. |
| APA | Fu, Yankai,Wu, Yanzhuo,Wei, Yuping,Chen, Xianhua,Xu, Jiandong,&Xu, Xia.(2015).Development of a thermally stable formulation for L-asparaginase storage in aqueous conditions.JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,122(DEC),8-14. |
| MLA | Fu, Yankai,et al."Development of a thermally stable formulation for L-asparaginase storage in aqueous conditions".JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 122.DEC(2015):8-14. |
入库方式： OAI收割
来源：过程工程研究所
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