Sensitivity of human embryonic stem cells to different conditions during cryopreservation
文献类型：期刊论文
| 作者 | Xu, Yanqing1; Zhang, Liang1,2; Xu, Jiandong1; Wei, Yuping1; Xu, Xia1 |
| 刊名 | CRYOBIOLOGY
 |
| 出版日期 | 2015-12-01 |
| 卷号 | 71期号:3页码:486-492 |
| 关键词 | hESCs Cryopreservation Recovery Ice formation Reactive oxidative species |
| ISSN号 | 0011-2240 |
| 英文摘要 | Low cell recovery rate of human embryonic stem cells (hESCs) resulting from cryopreservation damages leads to the difficulty in their successful commercialization of clinical applications. Hence in this study, sensitivity of human embryonic stem cells (hESCs) to different cooling rates, ice seeding and cryoprotective agent (CPA) types was compared and cell viability and recovery after cryopreservation under different cooling conditions were assessed. Both extracellular and intracellular ice formation were observed. Reactive oxidative species (ROS) accumulation of hESCs was determined. Cryopreservation of hESCs at 1 degrees C/min with the ice seeding and at the theoretically predicted optimal cooling rate (TPOCR) led to lower level of intracellular ROS, and prevented irregular and big ice clump formation compared with cryopreservation at 1 degrees C/min. This strategy further resulted in a significant increase in the hESC recovery when glycerol and 1,2-propanediol were used as the CPAs, but no increase for Me2SO. hESCs after cryopreservation under all the tested conditions still maintained their pluripotency. Our results provide guidance for improving the hESC cryopreservation recovery through the combination of CPA type, cooling rate and ice seeding. (C) 2015 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biology ; Physiology |
| 研究领域[WOS] | Life Sciences & Biomedicine - Other Topics ; Physiology |
| 关键词[WOS] | MEMBRANE-PERMEABILITY ; INTRACELLULAR ICE ; FREEZING INJURY ; ROCK INHIBITOR ; HUMAN OOCYTES ; SURVIVAL ; PROTOCOLS ; APOPTOSIS ; KINETICS ; RECOVERY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000367496800016 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/20252]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Yanqing,Zhang, Liang,Xu, Jiandong,et al. Sensitivity of human embryonic stem cells to different conditions during cryopreservation[J]. CRYOBIOLOGY,2015,71(3):486-492. |
| APA | Xu, Yanqing,Zhang, Liang,Xu, Jiandong,Wei, Yuping,&Xu, Xia.(2015).Sensitivity of human embryonic stem cells to different conditions during cryopreservation.CRYOBIOLOGY,71(3),486-492. |
| MLA | Xu, Yanqing,et al."Sensitivity of human embryonic stem cells to different conditions during cryopreservation".CRYOBIOLOGY 71.3(2015):486-492. |
入库方式： OAI收割
来源：过程工程研究所
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