Preparation and characterization of magnetic poly(styrene-glycidyl methacrylate) microspheres for highly efficient protein adsorption by two-stage dispersion polymerization
文献类型：期刊论文
| 作者 | Zhao, Xinwei1; Guan, Yueping1; Xia, Changfu1; Xia, Tingting2; Qiu, Xiaolin1; Wang, Chuhang1; Guo, Chen2 |
| 刊名 | JOURNAL OF APPLIED POLYMER SCIENCE
 |
| 出版日期 | 2016-03-10 |
| 卷号 | 133期号:10页码:43005 |
| 关键词 | adsorption functionalization of polymers properties and characterization proteins |
| ISSN号 | 0021-8995 |
| 英文摘要 | Micrometer-sized superparamagnetic poly(styrene-glycidyl methacrylate)/Fe3O4 spheres were synthesized by two-stage dispersion polymerization with modified hydrophobic Fe3O4 nanoparticles, styrene (St), and glycidyl methacrylate (GMA). The morphology and properties of the magnetic Fe3O4-P (St-GMA) microspheres were examined by scanning electron microscopy, transmission electron microscopy, vibrating sample magnetometry, thermogravimetric analysis, and attenuated total reflectance. The average size of the obtained magnetic microspheres was 1.50 mu m in diameter with a narrow size distribution, and the saturation magnetization of the magnetic microspheres was 8.23 emu/g. The magnetic Fe3O4-P (St-GMA) microspheres with immobilized iminodiacetic acid-Cu2+ groups were used to investigate the adsorption capacity and selectivity of the model proteins, bovine hemoglobin (BHb) and bovine serum albumin (BSA). We found that the adsorption capacity of BHb was as high as 190.66 mg/g of microspheres, which was 3.20 times greater than that of BSA, which was only 59.64 mg/g of microspheres as determined by high-performance liquid chromatography. With a rather low nonspecific adsorption, these microspheres have great potential for protein separation and purification applications. (C) 2015 Wiley Periodicals, Inc. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Polymer Science |
| 研究领域[WOS] | Polymer Science |
| 关键词[WOS] | EMULSION POLYMERIZATION ; MONODISPERSE ; PARTICLES ; NANOPARTICLES ; SEPARATION ; IMMOBILIZATION ; PURIFICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000367852900003 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/20260]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Lab Separat Sci & Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Xinwei,Guan, Yueping,Xia, Changfu,et al. Preparation and characterization of magnetic poly(styrene-glycidyl methacrylate) microspheres for highly efficient protein adsorption by two-stage dispersion polymerization[J]. JOURNAL OF APPLIED POLYMER SCIENCE,2016,133(10):43005. |
| APA | Zhao, Xinwei.,Guan, Yueping.,Xia, Changfu.,Xia, Tingting.,Qiu, Xiaolin.,...&Guo, Chen.(2016).Preparation and characterization of magnetic poly(styrene-glycidyl methacrylate) microspheres for highly efficient protein adsorption by two-stage dispersion polymerization.JOURNAL OF APPLIED POLYMER SCIENCE,133(10),43005. |
| MLA | Zhao, Xinwei,et al."Preparation and characterization of magnetic poly(styrene-glycidyl methacrylate) microspheres for highly efficient protein adsorption by two-stage dispersion polymerization".JOURNAL OF APPLIED POLYMER SCIENCE 133.10(2016):43005. |
入库方式： OAI收割
来源：过程工程研究所
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