Gas-assisted magnetic separation for the purification of proteins in batch systems
文献类型：期刊论文
| 作者 | Liu, Zhini1,2; Yang, Liangrong2; Dong, Tingting2; Li, Wensong1,2; Sun, Xitong2; Zhu, Menghao2; Duan, Zhengkang1; Liu, Qingfen2; Liu, Huizhou2 |
| 刊名 | PARTICUOLOGY
 |
| 出版日期 | 2016-02-01 |
| 卷号 | 24期号:FEB页码:170-176 |
| ISSN号 | 1674-2001 |
| 关键词 | Adsorption Magnetic separation Flotation Recovery BSA conformation |
| 英文摘要 | In this paper, gas-assisted magnetic separation (GAMS), a technique that combines magnetic separation with flotation, was investigated for the potential large-scale separation of proteins. The GAMS process includes adsorption of target proteins and magnetic separation to recover protein-loaded magnetic particles from the dilute biosuspension with the assistance of bubbles. Microsized ethylenediamine-functionalized poly(glycidyl methacrylate) superparamagnetic microspheres (MPMs) and bovine serum albumin (BSA) were used as a model system. The feasibility of GAMS for capturing BSA-loaded MPMs from an appropriate medium was shown. High recovery of BSA-loaded MPMs was obtained by simple adjustment of the initial solution pH without extra detergents and antifoaming agents. The GAMS conditions were consistent with the adsorption conditions, and no proteins were desorbed from the MPMs during this process. Under the optimal conditions, the separation rate and recovery percentage reached 410 mL/min and 98% in 0.61 min, respectively. Conformational changes of BSA during the GAMS process were investigated by fluorescence spectroscopy and circular dichroism spectrometry. (C) 2015 Chinese Society of Particuology and Institute of Process Engineering, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Technology |
| 类目[WOS] | Engineering, Chemical ; Materials Science, Multidisciplinary |
| 研究领域[WOS] | Engineering ; Materials Science |
| 关键词[WOS] | SUPERPARAMAGNETIC EXTRACTION ; ADSORPTION ; PARTICLES ; ADSORBENT ; FLOTATION ; NANOPARTICLES ; MICROSPHERES ; REMOVAL ; ALBUMIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000369457900020 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/20606]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Xiangtan Univ, Coll Chem Engn, Xiangtan 411105, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Zhini,Yang, Liangrong,Dong, Tingting,et al. Gas-assisted magnetic separation for the purification of proteins in batch systems[J]. PARTICUOLOGY,2016,24(FEB):170-176. |
| APA | Liu, Zhini.,Yang, Liangrong.,Dong, Tingting.,Li, Wensong.,Sun, Xitong.,...&Liu, Huizhou.(2016).Gas-assisted magnetic separation for the purification of proteins in batch systems.PARTICUOLOGY,24(FEB),170-176. |
| MLA | Liu, Zhini,et al."Gas-assisted magnetic separation for the purification of proteins in batch systems".PARTICUOLOGY 24.FEB(2016):170-176. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。