Roles of structural plasticity in chaperone HdeA activity are revealed by F-19 NMR
文献类型：期刊论文
| 作者 | Zhai, Zining1,2; Wu, Qiong1; Zheng, Wenwen1,2; Liu, Maili1; Pielak, Gary J.3,4,5; Li, Conggang1 |
| 刊名 | CHEMICAL SCIENCE
 |
| 出版日期 | 2016 |
| 卷号 | 7期号:3页码:2222-2228 |
| 英文摘要 | HdeA, a minimal ATP-independent acid chaperone, is crucial for the survival of enteric pathogens as they transit the acidic (pH 1-3) environment of the stomach. Although protein disorder (unfolding) and structural plasticity have been elegantly linked to HdeA function, the details of the linkage are lacking. Here, we apply F-19 NMR to reveal the structural transition associated with activation. We find that unfolding is necessary but not sufficient for activation. Multiple conformations are present in the functional state at low pH, but the partially folded conformation is essential for HdeA chaperone activity, and HdeA's intrinsic disulfide bond is required to maintain the partially folded conformation. The results show that both disorder and order are key to function. The ability of F-19 NMR to reveal and quantify multiple conformational states makes it a powerful tool for studying other chaperones. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Multidisciplinary |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | ACID RESISTANCE ; ESCHERICHIA-COLI ; ENTERIC BACTERIA ; PERIPLASMIC PROTEIN ; SUBSTRATE-BINDING ; SPECTROSCOPY ; CONFORMATION ; ACTIVATION ; DOMAIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000371021900075 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/9221]  |
| 专题 | 武汉物理与数学研究所_磁共振基础研究部 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Natl Ctr Magnet Resonance Wuhan,Wuhan Inst Phys &, Wuhan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Univ N Carolina, Dept Chem, Chapel Hill, NC USA 4.Univ N Carolina, Dept Biochem & Biophys, Chapel Hill, NC USA 5.Univ N Carolina, Lineberger Comprehens Canc Ctr, Chapel Hill, NC 27599 USA |
| 推荐引用方式 GB/T 7714 | Zhai, Zining,Wu, Qiong,Zheng, Wenwen,et al. Roles of structural plasticity in chaperone HdeA activity are revealed by F-19 NMR[J]. CHEMICAL SCIENCE,2016,7(3):2222-2228. |
| APA | Zhai, Zining,Wu, Qiong,Zheng, Wenwen,Liu, Maili,Pielak, Gary J.,&Li, Conggang.(2016).Roles of structural plasticity in chaperone HdeA activity are revealed by F-19 NMR.CHEMICAL SCIENCE,7(3),2222-2228. |
| MLA | Zhai, Zining,et al."Roles of structural plasticity in chaperone HdeA activity are revealed by F-19 NMR".CHEMICAL SCIENCE 7.3(2016):2222-2228. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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