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A single adaptive point mutation in Japanese encephalitis virus capsid is sufficient to render the virus as a stable vector for gene delivery

文献类型：期刊论文

作者Jia, Fan1,2; Zhu, Xutao1,2,3; Xu, Fuqiang1,2,4
刊名VIROLOGY
出版日期2016-03-01
卷号490页码:109-118
关键词Japanese encephalitis virus Adaptive mutation Stable vector Capsid Gene delivery
英文摘要Japanese encephalitis virus (JEV) is a neurotropic flavivirus that has broad range of hosts. Stable JEV vector has not been reported yet. Here, we constructed a JEV-EGFP by inserting a fragment of 08 (the N-terminal 38 amino acids of capsid)-EGFP-FMDV2A into the junction between 5'UTR and the N-terminus of capsid gene. An adaptive nucleotide mutation T45G (location at the N-terminus of capsid gene), resulting in an amino acid change from asparagine to lysine (N15K), was identified by genome sequencing. It stabilized the vector and enlarged the virion. The stabilizing effect might be general because it is also stable when EGFP was replaced with another marker, SNAP. A model was proposed for this stabilization effect based on previously published and our data. This finding may be used to construct various JEV-based stable delivery systems for virological studies and neural circuit tracing. (C) 2016 Elsevier Inc. All rights reserved.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Virology
研究领域[WOS]Virology
关键词[WOS]WEST-NILE-VIRUS ; GREEN FLUORESCENT PROTEIN ; CENTRAL-NERVOUS-SYSTEM ; YELLOW-FEVER-VIRUS ; DENGUE VIRUS ; PSEUDORABIES VIRUS ; RNA REPLICATION ; CODING REGION ; CORE PROTEIN ; IN-VITRO
收录类别SCI
语种英语
WOS记录号WOS:000371445400013
公开日期2016-05-03
源URL[http://ir.wipm.ac.cn/handle/112942/9220]  
专题武汉物理与数学研究所_磁共振应用研究部
作者单位1.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China
2.Chinese Acad Sci, Brain Res Ctr, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Ctr Excellence Brain Sci & Intelligence Technol, Shanghai 200031, Peoples R China
推荐引用方式
GB/T 7714		 Jia, Fan,Zhu, Xutao,Xu, Fuqiang. A single adaptive point mutation in Japanese encephalitis virus capsid is sufficient to render the virus as a stable vector for gene delivery[J]. VIROLOGY,2016,490:109-118.
APA Jia, Fan,Zhu, Xutao,&Xu, Fuqiang.(2016).A single adaptive point mutation in Japanese encephalitis virus capsid is sufficient to render the virus as a stable vector for gene delivery.VIROLOGY,490,109-118.
MLA Jia, Fan,et al."A single adaptive point mutation in Japanese encephalitis virus capsid is sufficient to render the virus as a stable vector for gene delivery".VIROLOGY 490(2016):109-118.

入库方式： OAI收割

来源：武汉物理与数学研究所

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