Separation of human serum albumin and polyethylene glycol by electro-ultrafiltration
文献类型：期刊论文
| 作者 | Chen, Guoqiang1,2; Song, Weijie1; Qi, Benkun1; Ghosh, Raja3; Wan, Yinhua1 |
| 刊名 | BIOCHEMICAL ENGINEERING JOURNAL
 |
| 出版日期 | 2016-05-15 |
| 卷号 | 109期号:MAY页码:127-136 |
| 关键词 | Ultrafiltration Electro-ultrafiltration Human serum albumin Polyethylene glycol Concentration polarization |
| ISSN号 | 1369-703X |
| 通讯作者 | Wan, YH (reprint author), Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China. |
| 英文摘要 | For the purpose of residual PEG removal after protein purification and PEGylation, electro-ultrafiltration (EUF) was used for separation of human serum albumin (HSA) from polyethylene glycol (PEG) with different molecular weights. The results clearly demonstrated the effectiveness of EUF for achieving the desired objectives. Higher flux and lower HSA transmission were observed in EUF process with the feed consisting of a mixture of 1 g/L PEG and 1 g/L HSA, compared to conventional ultrafiltration (UF) process, while the PEG transmission was nearly the same. When the concentration of PEG increased from 1 g/L to 10 g/L while keeping the HSA concentration the same, more HSA passed through the membrane in the UF process due to reduction in protein diffusion and hydration by PEG. When the HSA concentration increased from 1 g/L to 10 g/L with the PEG concentration being 1 g/L, the PEG transmission decreased significantly. The application of electric field resulted in high flux and PEG transmission and low protein transmission through the membrane. The effect of electrolyte (i.e., ionic strength and type) on the efficiency of the EUF process was also investigated. (C) 2016 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Engineering |
| 关键词[WOS] | CROSS-FLOW ULTRAFILTRATION ; AQUEOUS 2-PHASE SYSTEMS ; ELECTRIC-FIELD ; POLY(ETHYLENE GLYCOL) ; FRUIT JUICE ; PROTEINS ; MEMBRANE ; REMOVAL ; ELECTROPHORESIS ; RETENTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000372387200016 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/20628]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.McMaster Univ, Dept Chem Engn, Hamilton, ON L8S 4L8, Canada |
| 推荐引用方式 GB/T 7714 | Chen, Guoqiang,Song, Weijie,Qi, Benkun,et al. Separation of human serum albumin and polyethylene glycol by electro-ultrafiltration[J]. BIOCHEMICAL ENGINEERING JOURNAL,2016,109(MAY):127-136. |
| APA | Chen, Guoqiang,Song, Weijie,Qi, Benkun,Ghosh, Raja,&Wan, Yinhua.(2016).Separation of human serum albumin and polyethylene glycol by electro-ultrafiltration.BIOCHEMICAL ENGINEERING JOURNAL,109(MAY),127-136. |
| MLA | Chen, Guoqiang,et al."Separation of human serum albumin and polyethylene glycol by electro-ultrafiltration".BIOCHEMICAL ENGINEERING JOURNAL 109.MAY(2016):127-136. |
入库方式： OAI收割
来源：过程工程研究所
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