Covalent immobilization of trypsin onto thermo-sensitive poly(N-isopropylacrylamide-co-acrylic acid) microspheres with high activity and stability
文献类型：期刊论文
| 作者 | Lai, Enping1,2; Wang, Yuxia2; Wei, Yi2; Li, Guang1; Ma, Guanghui2 |
| 刊名 | JOURNAL OF APPLIED POLYMER SCIENCE
 |
| 出版日期 | 2016-06-05 |
| 卷号 | 133期号:21页码:43343 |
| 关键词 | bioengineering emulsion polymerization gels stimuli-sensitive polymers |
| ISSN号 | 0021-8995 |
| 通讯作者 | Li, G (reprint author), Donghua Univ, Coll Mat Sci & Engn, State Key Lab Modificat Chem Fibers & Polymer Mat, Shanghai 201620, Peoples R China. |
| 英文摘要 | Poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAM-co-AA)) microspheres with a high copolymerized AA content were fabricated using rapid membrane emulsification technique. The uniform size, good hydrophilicity, and thermo sensitivity of the microspheres were favorable for trypsin immobilization. Trypsin molecules were immobilized onto the microspheres surfaces by covalent attachment. The effects of various parameters such as immobilization pH value, enzyme concentration, concentration of buffer solution, and immobilization time on protein loading amount and enzyme activity were systematically investigated. Under the optimum conditions, the protein loading was 493 +/- 20 mgg(-1) and the activity yield of immobilized trypsin was 155%+/- 3%. The maximum activity (V-max) and Michaelis constant (K-m) of immobilized enzyme were found to be 0.74 Ms-1 and 0.54 mM, respectively. The immobilized trypsin showed better thermal and storage stability than the free trypsin. The enzyme-immobilized microspheres with high protein loading amount still can show a thermo reversible phase transition behavior. The research could provide a strategy to immobilize enzyme for application in proteomics. (c) 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 43343. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Polymer Science |
| 研究领域[WOS] | Polymer Science |
| 关键词[WOS] | SUBSEQUENT SUSPENSION POLYMERIZATION ; ENZYME-IMMOBILIZATION ; EMULSIFICATION TECHNIQUE ; PROTEIN-ANALYSIS ; TEMPERATURE ; PROTEOMICS ; MICROGELS ; HYDROGEL ; NANOPARTICLES ; PURIFICATION |
| 收录类别 | SCI |
| 原文出处 | WILEY-BLACKWELL |
| 语种 | 英语 |
| WOS记录号 | WOS:000371249800001 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/20643]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Donghua Univ, Coll Mat Sci & Engn, State Key Lab Modificat Chem Fibers & Polymer Mat, Shanghai 201620, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lai, Enping,Wang, Yuxia,Wei, Yi,et al. Covalent immobilization of trypsin onto thermo-sensitive poly(N-isopropylacrylamide-co-acrylic acid) microspheres with high activity and stability[J]. JOURNAL OF APPLIED POLYMER SCIENCE,2016,133(21):43343. |
| APA | Lai, Enping,Wang, Yuxia,Wei, Yi,Li, Guang,&Ma, Guanghui.(2016).Covalent immobilization of trypsin onto thermo-sensitive poly(N-isopropylacrylamide-co-acrylic acid) microspheres with high activity and stability.JOURNAL OF APPLIED POLYMER SCIENCE,133(21),43343. |
| MLA | Lai, Enping,et al."Covalent immobilization of trypsin onto thermo-sensitive poly(N-isopropylacrylamide-co-acrylic acid) microspheres with high activity and stability".JOURNAL OF APPLIED POLYMER SCIENCE 133.21(2016):43343. |
入库方式： OAI收割
来源：过程工程研究所
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