High-throughput screening of high Monascus pigment-producing strain based on digital image processing
文献类型：期刊论文
| 作者 | Xia, Meng-lei1,2; Wang, Lan1; Yang, Zhi-xia3; Chen, Hong-zhang1
|
| 刊名 | JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
 |
| 出版日期 | 2016-04-01 |
| 卷号 | 43期号:4页码:451-461 |
| 关键词 | Monascus ruber Pigment Image processing Support vector machine Automatized operation |
| ISSN号 | 1367-5435 |
| 通讯作者 | Chen, HZ (reprint author), Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China. |
| 英文摘要 | This work proposed a new method which applied image processing and support vector machine (SVM) for screening of mold strains. Taking Monascus as example, morphological characteristics of Monascus colony were quantified by image processing. And the association between the characteristics and pigment production capability was determined by SVM. On this basis, a highly automated screening strategy was achieved. The accuracy of the proposed strategy is 80.6 %, which is compatible with the existing methods (81.1 % for microplate and 85.4 % for flask). Meanwhile, the screening of 500 colonies only takes 20-30 min, which is the highest rate among all published results. By applying this automated method, 13 strains with high-predicted production were obtained and the best one produced as 2.8-fold (226 U/mL) of pigment and 1.9-fold (51 mg/L) of lovastatin compared with the parent strain. The current study provides us with an effective and promising method for strain improvement. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | SUPPORT VECTOR MACHINE ; SUBMERGED FERMENTATION ; MORPHOLOGY ; ASPERGILLUS ; PURPUREUS ; CULTURE |
| 收录类别 | SCI |
| 原文出处 | SPRINGER HEIDELBERG |
| 语种 | 英语 |
| WOS记录号 | WOS:000372538900003 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/20651]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Xinjiang Univ, Coll Math & Syst Sci, Urumqi 830046, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xia, Meng-lei,Wang, Lan,Yang, Zhi-xia,et al. High-throughput screening of high Monascus pigment-producing strain based on digital image processing[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2016,43(4):451-461. |
| APA | Xia, Meng-lei,Wang, Lan,Yang, Zhi-xia,&Chen, Hong-zhang.(2016).High-throughput screening of high Monascus pigment-producing strain based on digital image processing.JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,43(4),451-461. |
| MLA | Xia, Meng-lei,et al."High-throughput screening of high Monascus pigment-producing strain based on digital image processing".JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 43.4(2016):451-461. |
入库方式： OAI收割
来源：过程工程研究所
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