High molecular weight beta-poly(L-malic acid) produced by A. pullulans with Ca2+ added repeated batch culture
文献类型：期刊论文
| 作者 | Cao, Weifeng1; Chen, Xiangrong1; Luo, Jianquan1; Yin, Junxiang2; Qiao, Changsheng3; Wan, Yinhua1 |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2016-04-01 |
| 卷号 | 85期号:APR页码:192-199 |
| 关键词 | beta-poly(L-malic acid) Aureobasidium pullulans Molecular weight |
| ISSN号 | 0141-8130 |
| 通讯作者 | Wan, YH (reprint author), Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China. |
| 英文摘要 | beta-Poly(malic acid) (PMLA) has attracted increasing attentions because of its potential application in medicine and other industries. In this study, the variation of PMLA molecular weight (M-w) in the batch culture and the strategies to enhance PMLA M-w were studied. Adding exogenous Ca2+ (0.1 g/L CaCl2) to the medium caused a significant increase in both PMLA concentration and M-w (11.38% and 26.3%, respectively) when Na2CO3 was used as the neutralizer. The M-w of PMLA during the process of batch culture, which associated with the specific PMLA production per unit cell mass (Y-p/x) before glucose was depleted, increased from 12.522KDa to its maximum 18.693KDa and then kept decreasing until the end of the culture. Compared with the results in batch culture, M-w increased by 84.4% (up to 19.51 kDa) with a productivity of 1.1 gh(-1) L-1 when the cells were maintained in exponential growth phase during Ca2+ added repeated batch culture. The present work provides an efficient approach to obtain superior quality PMLA product with high M-w. (C) 2015 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 学科主题 | Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science ; Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 类目[WOS] | Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 关键词[WOS] | FUNGUS AUREOBASIDIUM-PULLULANS ; POLY-L-MALATE ; L-MALIC ACID ; PHYSARUM-POLYCEPHALUM ; POLYMALIC ACID ; SACCHAROMYCES-CEREVISIAE ; MELANIN ; STRAIN ; FERMENTATION ; COMBINATION |
| 收录类别 | SCI |
| 原文出处 | ELSEVIER SCIENCE BV |
| 语种 | 英语 |
| WOS记录号 | WOS:000370886800022 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/20692]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.China Natl Ctr Biotechnol Dev, Beijing 100036, Peoples R China 3.Tianjin Univ Sci & Technol, Dept Bioengn, Tianjin 300457, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Weifeng,Chen, Xiangrong,Luo, Jianquan,et al. High molecular weight beta-poly(L-malic acid) produced by A. pullulans with Ca2+ added repeated batch culture[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2016,85(APR):192-199. |
| APA | Cao, Weifeng,Chen, Xiangrong,Luo, Jianquan,Yin, Junxiang,Qiao, Changsheng,&Wan, Yinhua.(2016).High molecular weight beta-poly(L-malic acid) produced by A. pullulans with Ca2+ added repeated batch culture.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,85(APR),192-199. |
| MLA | Cao, Weifeng,et al."High molecular weight beta-poly(L-malic acid) produced by A. pullulans with Ca2+ added repeated batch culture".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 85.APR(2016):192-199. |
入库方式： OAI收割
来源：过程工程研究所
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