Sensing for intracellular thiols by water-insoluble two-photon fluorescent probe incorporating nanogel
文献类型：期刊论文
| 作者 | Guo, Xudong1; Zhang, Xin1; Wang, Shuangqing1; Li, Shayu1; Hu, Rui1; Li, Yi2; Yang, Guoqiang1 |
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2015-04-15 |
| 卷号 | 869页码:81-88 |
| 关键词 | Sensing Intracellular thiols Two-photon fluorescent probe Water-insoluble Nanogel |
| 英文摘要 | A novel "turn-on" two-photon fluorescent probe containing a p-conjugated triarylboron luminogen and a maleimide moiety DMDP-M based on the photo-induced electron transfer (PET) mechanism for biothiol detection was designed and synthesized. By simply loading the hydrophobic DMDP-M on a crosslinked Pluronic (R) F127 nanogel (CL-F127), a probing system DMDP-M/CL-F127 was established, which shows quick response, high selectivity and sensitivity to cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) in aqueous phase. The DMDP-M/CL-F127 system presented the fastest response to Cys with a rate constant of 0.56 min(-1), and the detection limit to Cys was calculated to be as low as 0.18 mu M. The DMDP-M/CL-F127 system has been successfully applied to the fluorescence imaging of biothiols in NIH/3T3 fibroblasts either with single-photon or two-photon excitation because of its high biocompatibility and cell-membrane permeability. The present work provides a general, simple and efficient strategy for the application of hydrophobic molecules to sensing biothiols in aqueous phase, and a novel sensing system for intracellular biothiols fitted for both single-photon and two-photon fluorescence imaging. (C) 2015 Elsevier B.V. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/28430]  |
| 专题 | 化学研究所_光化学实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Key Lab Photochem, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Tech Inst Phys & Chem, Key Lab Photochem Convers & Optoelect Mat, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Xudong,Zhang, Xin,Wang, Shuangqing,et al. Sensing for intracellular thiols by water-insoluble two-photon fluorescent probe incorporating nanogel[J]. ANALYTICA CHIMICA ACTA,2015,869:81-88. |
| APA | Guo, Xudong.,Zhang, Xin.,Wang, Shuangqing.,Li, Shayu.,Hu, Rui.,...&Yang, Guoqiang.(2015).Sensing for intracellular thiols by water-insoluble two-photon fluorescent probe incorporating nanogel.ANALYTICA CHIMICA ACTA,869,81-88. |
| MLA | Guo, Xudong,et al."Sensing for intracellular thiols by water-insoluble two-photon fluorescent probe incorporating nanogel".ANALYTICA CHIMICA ACTA 869(2015):81-88. |
入库方式： OAI收割
来源：化学研究所
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