中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 化学研究所
  3. 中国科学院化学研究所
  4. 光化学实验室
Intracellular Fluorescent Temperature Probe Based on Triarylboron Substituted Poly N-Isopropylacrylamide and Energy Transfer

文献类型：期刊论文

作者Liu, Jun1; Guo, Xudong1; Hu, Rui1; Xu, Jian1; Wang, Shuangqing1; Li, Shayu1; Li, Yi2; Yang, Guoqiang1
刊名ANALYTICAL CHEMISTRY
出版日期2015-04-07
卷号87期号:7页码:3694-3698
英文摘要A novel hydrophilic fluorescence temperature probe (PNDP) based on polarity-sensitive triarylboron compound (DPTB) and PNIPAM is designed and synthesized. In order to overcome the shortcomings of the single-intensity-based sensing mechanism and obtain more robust signals, ratiometric readout is achieved by designing an efficient FRET system (PNDP-NR) between DPTB and Nile Red (NR). PNDP-NR possesses some excellent features, including wide temperature range, good linear relationship, high temperature resolution, excellent reversibility, and stability. Within a sensing temperature range of 30-55 degrees C, the fluorescence color of PNDP-NR experiences significant change from red to green-blue. PNDP-NR is also introduced into NIH/3T3 cells to sense the temperature at the single-cell level. It gave excellent photostability and low cytotoxicity in vivo.
收录类别SCI
语种英语
公开日期2016-05-09
源URL[http://ir.iccas.ac.cn/handle/121111/28535]  
专题化学研究所_光化学实验室
作者单位1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Key Lab Photochem, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Key Lab Photochem Convers & Optoelect Mat, Tech Inst Phys & Chem, Beijing 100190, Peoples R China
推荐引用方式
GB/T 7714		 Liu, Jun,Guo, Xudong,Hu, Rui,et al. Intracellular Fluorescent Temperature Probe Based on Triarylboron Substituted Poly N-Isopropylacrylamide and Energy Transfer[J]. ANALYTICAL CHEMISTRY,2015,87(7):3694-3698.
APA Liu, Jun.,Guo, Xudong.,Hu, Rui.,Xu, Jian.,Wang, Shuangqing.,...&Yang, Guoqiang.(2015).Intracellular Fluorescent Temperature Probe Based on Triarylboron Substituted Poly N-Isopropylacrylamide and Energy Transfer.ANALYTICAL CHEMISTRY,87(7),3694-3698.
MLA Liu, Jun,et al."Intracellular Fluorescent Temperature Probe Based on Triarylboron Substituted Poly N-Isopropylacrylamide and Energy Transfer".ANALYTICAL CHEMISTRY 87.7(2015):3694-3698.

入库方式： OAI收割

来源：化学研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。