Type I Interferons Function as Autocrine and Paracrine Factors to Induce Autotaxin in Response to TLR Activation
文献类型：期刊论文
| 作者 | Song, Jianwen1; Guan, Ming2; Zhao, Zhenwen2; Zhang, Junjie1 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2015-08-27 |
| 卷号 | 10期号:8 |
| 英文摘要 | Lysophosphatidic acid (LPA) is an important phospholipid mediator in inflammation and immunity. However, the mechanism of LPA regulation during inflammatory response is largely unknown. Autotaxin (ATX) is the key enzyme to produce extracellular LPA from lysophosphatidylcholine (LPC). In this study, we found that ATX was induced in monocytic THP-1 cells by TLR4 ligand lipopolysaccharide (LPS), TLR9 ligand CpG oligonucleotide, and TLR3 ligand poly(I:C), respectively. The ATX induction by TLR ligand was abolished by the neutralizing antibody against IFN-beta or the knockdown of IFNAR1, indicating that type I IFN autocrine loop is responsible for the ATX induction upon TLR activation. Both IFN-beta and IFN-alpha were able to induce ATX expression via the JAK-STAT and PI3K-AKT pathways but with different time-dependent manners. The ATX induction by IFN-beta was dramatically enhanced by IFN-gamma, which had no significant effect on ATX expression alone, suggesting a synergy effect between type I and type II IFNs in ATX induction. Extracellular LPA levels were significantly increased when THP-1 cells were treated with IFN-alpha/beta or TLR ligands. In addition, the type I IFN-mediated ATX induction was identified in human monocyte-derived dendritic cells (moDCs) stimulated with LPS or poly(I: C), and IFN-alpha/beta could induce ATX expression in human peripheral blood mononuclear cells (PBMCs) and monocytes isolated form blood samples. These results suggest that, in response to TLR activation, ATX is induced through a type I INF autocrine-paracrine loop to enhance LPA generation. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/27685]  |
| 专题 | 化学研究所_活体分析化学实验室 |
| 作者单位 | 1.Beijing Normal Univ, Coll Life Sci, Inst Cell Biol, Key Lab Cell Proliferat & Regulat Biol,Minist Edu, Beijing 100875, Peoples R China 2.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Jianwen,Guan, Ming,Zhao, Zhenwen,et al. Type I Interferons Function as Autocrine and Paracrine Factors to Induce Autotaxin in Response to TLR Activation[J]. PLOS ONE,2015,10(8). |
| APA | Song, Jianwen,Guan, Ming,Zhao, Zhenwen,&Zhang, Junjie.(2015).Type I Interferons Function as Autocrine and Paracrine Factors to Induce Autotaxin in Response to TLR Activation.PLOS ONE,10(8). |
| MLA | Song, Jianwen,et al."Type I Interferons Function as Autocrine and Paracrine Factors to Induce Autotaxin in Response to TLR Activation".PLOS ONE 10.8(2015). |
入库方式： OAI收割
来源：化学研究所
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