Simultaneous determination of electrophoretic and dielectrophoretic mobilities of human red blood cells
文献类型：期刊论文
| 作者 | Zhu, Xiuzhen1; Chen, Yi1,2 |
| 刊名 | AMINO ACIDS
 |
| 出版日期 | 2015-08-01 |
| 卷号 | 47期号:8页码:1673-1674 |
| 关键词 | Dielectrophoretic and electrophoretic mobilities Human red blood cells Simultaneous determination |
| 英文摘要 | Electrophoresis and dielectrophoresis of cells can reveal many distinct cellular properties but are often conducted separately. Herein a simultaneous strategy was proposed, and a simple method was established by making cells migrate through a cross channel under a micro video for real-time observation. The experiment can be performed within 0.044-1 s. In combination with digital calculation based on electromagnetic theory, the method was validated to be applicable to the determination of electrophoretic and dielectrophoretic mobilities, mu(EP) and mu(DEP), of human blood erythrocytes, giving mu(EP) = -(0.87 +/- 0.16) x10(-4) cm(2).V-1.s(-1) and mu(DEP)=-(4.5 +/- 1.3) x10(-8) cm(4).V-2.s(-1) by vector decomposition, or mu(EP)=-(0.89 +/- 0.14) x10(-4) cm(2).V-1.s(-1) and mu(DEP) =-(4.6 +/- 1.2) x10(-8) cm(4).V-2.s(-1) by least squares fitting, all agreeing with published data. Hydrodynamic and EOFs were eliminated for better measurement. It was found that the location of cells had a serious impact on the measurement precision, and the upstream of the cross channel along the electric field was chosen for precise measurement. The method is also extendable to the study of other cells and particles. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/27844]  |
| 专题 | 化学研究所_活体分析化学实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China 2.Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Xiuzhen,Chen, Yi. Simultaneous determination of electrophoretic and dielectrophoretic mobilities of human red blood cells[J]. AMINO ACIDS,2015,47(8):1673-1674. |
| APA | Zhu, Xiuzhen,&Chen, Yi.(2015).Simultaneous determination of electrophoretic and dielectrophoretic mobilities of human red blood cells.AMINO ACIDS,47(8),1673-1674. |
| MLA | Zhu, Xiuzhen,et al."Simultaneous determination of electrophoretic and dielectrophoretic mobilities of human red blood cells".AMINO ACIDS 47.8(2015):1673-1674. |
入库方式： OAI收割
来源：化学研究所
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