HOCl can appear in the mitochondria of macrophages during bacterial infection as revealed by a sensitive mitochondrial-targeting fluorescent probe
文献类型：期刊论文
| 作者 | Zhou, Jin; Li, Lihong; Shi, Wen; Gao, Xinghui; Li, Xiaohua; Ma, Huimin |
| 刊名 | CHEMICAL SCIENCE
 |
| 出版日期 | 2015 |
| 卷号 | 6期号:8页码:4884-4888 |
| 英文摘要 | Macrophages, important cells of the innate immune system, can produce abundant HOCl in the cytoplasm to fight against bacteria. Recent studies suggest that mitochondria in macrophages play a role in antibacterial responses. During bacterial infection, however, it is uncertain whether HOCl is present in the mitochondria, mainly because of the lack of a suitable research method. Herein, by developing a new mitochondrial-targeting fluorescent HOCl probe, combined with confocal fluorescence imaging, we show for the first time that HOCl can appear in the mitochondria of macrophages (Raw264.7 cells) during bacterial infection, as confirmed with non-phagocytic cells and inhibitors as control experiments. Moreover, the developed probe exhibits an accurate mitochondrial-targeting ability, a fast response, and high selectivity and sensitivity (detection limit 9 nM), and is thus expected to be employed for further revealing the biological function of subcellular mitochondria. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/27851]  |
| 专题 | 化学研究所_活体分析化学实验室 |
| 作者单位 | Chinese Acad Sci, Beijing Natl Lab Mol Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Jin,Li, Lihong,Shi, Wen,et al. HOCl can appear in the mitochondria of macrophages during bacterial infection as revealed by a sensitive mitochondrial-targeting fluorescent probe[J]. CHEMICAL SCIENCE,2015,6(8):4884-4888. |
| APA | Zhou, Jin,Li, Lihong,Shi, Wen,Gao, Xinghui,Li, Xiaohua,&Ma, Huimin.(2015).HOCl can appear in the mitochondria of macrophages during bacterial infection as revealed by a sensitive mitochondrial-targeting fluorescent probe.CHEMICAL SCIENCE,6(8),4884-4888. |
| MLA | Zhou, Jin,et al."HOCl can appear in the mitochondria of macrophages during bacterial infection as revealed by a sensitive mitochondrial-targeting fluorescent probe".CHEMICAL SCIENCE 6.8(2015):4884-4888. |
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来源：化学研究所
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