Functional analysis of synthetic DELLA domain peptides and bioactive gibberellin assay using surface plasmon resonance technology
文献类型：期刊论文
| 作者 | Zhao, Zhuoya1; Xing, Zenan1; Zhou, Min1,2; Chen, Yi3; Li, Chenzhong4; Wang, Ruozhong2; Xu, Wenzhong1; Ma, Mi1 |
| 刊名 | TALANTA
 |
| 出版日期 | 2015-11-01 |
| 卷号 | 144页码:502-509 |
| 关键词 | Bioactive gibberellin DELLA region Gibberellin receptor Qualitative and quantitative analysis SPR Synthetic peptide |
| 英文摘要 | DELLA proteins and phytohormone gibberellin act together to control convergence point of plant development. A gibberellin-bound nuclear receptor that interacts with the N-terminal domain of DELLA proteins is required for gibberellin induced degradation of DELLA proteins. N-terminal DELLA domain includes two conserved motifs: DELLA and VHYNP. However, their respective functions remain unclear. Meanwhile, the identification and detection of several bioactive gibberellins from the more than 100 gibberellin metabolites are overwhelmingly difficult for their similar structures. Using in vitro biochemical approach, our work demonstrates for the first time that the synthetic GAI N-terminal DELLA domain peptides have similar bioactive function as the expressed protein to interact with AtGID1 a receptor. Furthermore, our results reveal that DELLA motif is vitally important region and DELLA segment is essentially required region to recognize AtGID1a receptor. Finally, based on bioactive GA-dependent of the interaction between AtGID1a and DELLA protein, we generated a new method that could identify and detect bioactive GAs accurately and rapidly with surface plasmon resonance assays. (C) 2015 Elsevier B.V. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/29172]  |
| 专题 | 化学研究所_活体分析化学实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth & Dev, Changsha 410128, Hunan, Peoples R China 3.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China 4.Florida Int Univ, Dept Biomed Engn, Nanobiosensors Bioelect Lab, Miami, FL 33174 USA |
| 推荐引用方式 GB/T 7714 | Zhao, Zhuoya,Xing, Zenan,Zhou, Min,et al. Functional analysis of synthetic DELLA domain peptides and bioactive gibberellin assay using surface plasmon resonance technology[J]. TALANTA,2015,144:502-509. |
| APA | Zhao, Zhuoya.,Xing, Zenan.,Zhou, Min.,Chen, Yi.,Li, Chenzhong.,...&Ma, Mi.(2015).Functional analysis of synthetic DELLA domain peptides and bioactive gibberellin assay using surface plasmon resonance technology.TALANTA,144,502-509. |
| MLA | Zhao, Zhuoya,et al."Functional analysis of synthetic DELLA domain peptides and bioactive gibberellin assay using surface plasmon resonance technology".TALANTA 144(2015):502-509. |
入库方式： OAI收割
来源：化学研究所
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