Functionalized Carbon Quantum Dots with Dopamine for Tyrosinase Activity Monitoring and Inhibitor Screening: In Vitro and Intracellular Investigation
文献类型：期刊论文
| 作者 | Chai, Lujing1; Zhou, Jin2; Feng, Hui1; Tang, Cong1; Huang, Yuanyuan1; Qian, Zhaosheng1 |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2015-10-28 |
| 卷号 | 7期号:42页码:23564-23574 |
| 关键词 | tyrosinase carbon quantum dots fluorometric assay inhibitor screening intracellular imaging |
| 英文摘要 | Sensitive assay of tyrosinase (TYR) activity is in urgent demand for both fundamental research and practical application, but the exploration of functional materials with good biocompatibility for its activity evaluation at the intracellular level is still challenging until now. In this work, we develop a convenient and real-time assay with high sensitivity for TYR activity/level monitoring and its inhibitor screening based on biocompatible dopamine functionalized carbon quantum dots (Dopa-CQDs). Dopamine with redox property was functionalized on the surface of carbon quantum dots to construct a Dopa-CQDs conjugate with strong bluish green fluorescence. When the dopamine moiety in Dopa-CQDs conjugate was oxidized to a dopaquinone derivative under specific catalysis of TYR, an intrapartide photoinduced electron transfer (PET) process between CQDs and dopaquinone moiety took place, and then the fluorescence of the conjugate could be quenched simultaneously. Quantitative evaluation of TYR activity was established in terms of the relationship between fluorescence quenching efficiency and TYR activity. The assay covered a broad linear range of up to 800 U/L with a low detection limit of 7.0 U/L. Arbutin, a typical inhibitor of TYR, was chosen as an example to assess its function of inhibitor screening, and positive results were observed that fluorescence quenching extent of the probe was reduced in the presence of arbutin. It is also demonstrated that Dopa-CQD conjugate possesses excellent biocompatibility, and can sensitively monitor intracellular tyrosinase level in melanoma cells and intracellular pH changes in living cells, which provides great potential in application of TYR/pH-associated disease monitoring and medical diagnostics. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/29184]  |
| 专题 | 化学研究所_活体分析化学实验室 |
| 作者单位 | 1.Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321004, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chai, Lujing,Zhou, Jin,Feng, Hui,et al. Functionalized Carbon Quantum Dots with Dopamine for Tyrosinase Activity Monitoring and Inhibitor Screening: In Vitro and Intracellular Investigation[J]. ACS APPLIED MATERIALS & INTERFACES,2015,7(42):23564-23574. |
| APA | Chai, Lujing,Zhou, Jin,Feng, Hui,Tang, Cong,Huang, Yuanyuan,&Qian, Zhaosheng.(2015).Functionalized Carbon Quantum Dots with Dopamine for Tyrosinase Activity Monitoring and Inhibitor Screening: In Vitro and Intracellular Investigation.ACS APPLIED MATERIALS & INTERFACES,7(42),23564-23574. |
| MLA | Chai, Lujing,et al."Functionalized Carbon Quantum Dots with Dopamine for Tyrosinase Activity Monitoring and Inhibitor Screening: In Vitro and Intracellular Investigation".ACS APPLIED MATERIALS & INTERFACES 7.42(2015):23564-23574. |
入库方式： OAI收割
来源：化学研究所
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