Sensitive electrochemical immunosensor for alpha-fetoprotein based on graphene/SnO2/Au nanocomposite
文献类型：期刊论文
| 作者 | Liu, Junfeng1; Lin, Guanhua2; Xiao, Can1; Xue, Ying1; Yang, Ankang1; Ren, Hongxuan3; Lu, Wensheng2; Zhao, Hong1; Li, Xiangjun1; Yuan, Zhuobin1 |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2015-09-15 |
| 卷号 | 71页码:82-87 |
| 关键词 | Electrochemical immunosensor alpha-Fetoprotein Graphene Nanocomposite |
| 英文摘要 | A label-free electrochemical immunosensor for sensitive detection of alpha-fetoprotein (AFP) was developed based on graphene/SnO2/Au nanocomposite. The graphene/SnO2/Au nanocomposite modified glassy carbon electrode was used to immobilize alpha-fetoprotein antibody (anti-AFP) and to construct the immunosensor. Results demonstrated that the peak currents of [Ru(NH3)(6)](3+) decreased due to the interaction between antibody and antigen on the modified electrode. Thus, a label-free immunosensor for the detection of AFP was realized by monitoring the peak current change of [Ru(NH3)(6)](3+). The factors influencing the performance of the immunosensor were investigated in details. Under optimal conditions, the peak currents obtained by DPV decreased linearly with the increasing AFP concentrations in the range from 0.02 to 50 ng mL(-1) with a linear coefficient of 0.9959. This electrochemical immunoassay has a low detection limit of 0.01 ng mL(-1) (S/N=3) and was successfully applied to the determination of AFP in serum samples. (C) 2015 Elsevier B.V. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/27961]  |
| 专题 | 化学研究所_胶体、界面与化学热力学实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Sch Chem & Chem Engn, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Chem, Key Lab Colloids & Surfaces, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 3.Natl Ctr Nanosci & Technol China, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Junfeng,Lin, Guanhua,Xiao, Can,et al. Sensitive electrochemical immunosensor for alpha-fetoprotein based on graphene/SnO2/Au nanocomposite[J]. BIOSENSORS & BIOELECTRONICS,2015,71:82-87. |
| APA | Liu, Junfeng.,Lin, Guanhua.,Xiao, Can.,Xue, Ying.,Yang, Ankang.,...&Yuan, Zhuobin.(2015).Sensitive electrochemical immunosensor for alpha-fetoprotein based on graphene/SnO2/Au nanocomposite.BIOSENSORS & BIOELECTRONICS,71,82-87. |
| MLA | Liu, Junfeng,et al."Sensitive electrochemical immunosensor for alpha-fetoprotein based on graphene/SnO2/Au nanocomposite".BIOSENSORS & BIOELECTRONICS 71(2015):82-87. |
入库方式： OAI收割
来源：化学研究所
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