SNSMIL, a real-time single molecule identification and localization algorithm for super-resolution fluorescence microscopy
文献类型：期刊论文
| 作者 | Tang, Yunqing1,2,3; Dai, Luru1; Zhang, Xiaoming1; Li, Junbai1,4; Hendriks, Johnny5; Fan, Xiaoming5; Gruteser, Nadine5; Meisenberg, Annika5; Baumann, Arnd5; Katranidis, Alexandros6 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2015-06-22 |
| 卷号 | 5 |
| 英文摘要 | Single molecule localization based super-resolution fluorescence microscopy offers significantly higher spatial resolution than predicted by Abbe's resolution limit for far field optical microscopy. Such super-resolution images are reconstructed from wide-field or total internal reflection single molecule fluorescence recordings. Discrimination between emission of single fluorescent molecules and background noise fluctuations remains a great challenge in current data analysis. Here we present a real-time, and robust single molecule identification and localization algorithm, SNSMIL (Shot Noise based Single Molecule Identification and Localization). This algorithm is based on the intrinsic nature of noise, i.e., its Poisson or shot noise characteristics and a new identification criterion, Q(SNSMIL), is defined. SNSMIL improves the identification accuracy of single fluorescent molecules in experimental or simulated datasets with high and inhomogeneous background. The implementation of SNSMIL relies on a graphics processing unit (GPU), making real-time analysis feasible as shown for real experimental and simulated datasets. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/27975]  |
| 专题 | 化学研究所_胶体、界面与化学热力学实验室 |
| 作者单位 | 1.Natl Ctr Nanosci & Technol China, Beijing 100190, Peoples R China 2.Chongqing Univ, Dept Phys, Chongqing 400044, Peoples R China 3.Chinese Acad Sci, Inst Theoret Phys, State Key Lab Theoret Phys, Beijing 100190, Peoples R China 4.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China 5.Forschungszentrum Julich, Inst Complex Syst, ICS Cellular Biophys 4, D-52428 Julich, Germany 6.Forschungszentrum Julich, Inst Complex Syst, ICS Mol Biophys 5, D-52428 Julich, Germany |
| 推荐引用方式 GB/T 7714 | Tang, Yunqing,Dai, Luru,Zhang, Xiaoming,et al. SNSMIL, a real-time single molecule identification and localization algorithm for super-resolution fluorescence microscopy[J]. SCIENTIFIC REPORTS,2015,5. |
| APA | Tang, Yunqing.,Dai, Luru.,Zhang, Xiaoming.,Li, Junbai.,Hendriks, Johnny.,...&Gensch, Thomas.(2015).SNSMIL, a real-time single molecule identification and localization algorithm for super-resolution fluorescence microscopy.SCIENTIFIC REPORTS,5. |
| MLA | Tang, Yunqing,et al."SNSMIL, a real-time single molecule identification and localization algorithm for super-resolution fluorescence microscopy".SCIENTIFIC REPORTS 5(2015). |
入库方式： OAI收割
来源：化学研究所
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