Ultrafast Photoinduced Electron Transfer in Green Fluorescent Protein Bearing a Genetically Encoded Electron Acceptor
文献类型：期刊论文
| 作者 | Lv, Xiaoxuan1,2; Yu, Yang4; Zhou, Meng3; Hu, Cheng1,2; Gao, Feng1,2; Li, Jiasong1,2; Liu, Xiaohong1,2; Deng, Kai1,2; Zheng, Peng1,2; Gong, Weimin1,2 |
| 刊名 | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
 |
| 出版日期 | 2015-06-17 |
| 卷号 | 137期号:23页码:7270-7273 |
| 英文摘要 | Electron transfer (ET) is widely used for driving the processes that underlie the chemistry of life. However, our abilities to probe electron transfer mechanisms in proteins and design redox enzymes are limited, due to :the lack of methods to site-specifically insert electron acceptors into proteins in vivo. Here we describe the synthesis and genetic incorporation of 4-fluoro-3-nitrophenylalanine (FNO(2)Phe), which has similar reduction potentials to NAD(P)H and ferredoxin, the most important biological reductants. Through the genetic incorporation of FNO2Phe into green fluorescent:protein (GFP) and femtosecond transient absorption measurement, we show that photoinduced electron transfer (PET) from the GFP chromophore to FNO2Phe occurs very fast (within 11 ps), which is comparable to that of the first electron transfer step in photosystem I, from P700* to A(0). This genetically encoded, low-reduction potential unnatural amino acid (UAA) can significantly in-Trove our ability to investigate electron transfer mechanisms in complex reductases and facilitate the design of miniature proteins that mimic their functions. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/27948]  |
| 专题 | 化学研究所_光化学实验室 |
| 作者单位 | 1.Chinese Acad Sci, Lab RNA Biol, Inst Biophys, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Lab Quantum Biophys, Inst Biophys, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China 4.Univ Illinois, Dept Chem, Urbana, IL 61801 USA |
| 推荐引用方式 GB/T 7714 | Lv, Xiaoxuan,Yu, Yang,Zhou, Meng,et al. Ultrafast Photoinduced Electron Transfer in Green Fluorescent Protein Bearing a Genetically Encoded Electron Acceptor[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2015,137(23):7270-7273. |
| APA | Lv, Xiaoxuan.,Yu, Yang.,Zhou, Meng.,Hu, Cheng.,Gao, Feng.,...&Wang, Jiangyun.(2015).Ultrafast Photoinduced Electron Transfer in Green Fluorescent Protein Bearing a Genetically Encoded Electron Acceptor.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,137(23),7270-7273. |
| MLA | Lv, Xiaoxuan,et al."Ultrafast Photoinduced Electron Transfer in Green Fluorescent Protein Bearing a Genetically Encoded Electron Acceptor".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 137.23(2015):7270-7273. |
入库方式： OAI收割
来源：化学研究所
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