Graphene-Oxide-Conjugated Polymer Hybrid Materials for Calmodulin Sensing by Using FRET Strategy
文献类型：期刊论文
| 作者 | Yuan, Hongbo1,2; Qi, Junjie1; Xing, Chengfen1; An, Hailong1; Niu, Ruimin1; Zhan, Yong1; Fan, Yibing1; Yan, Wenmin1; Li, Ruihua1; Wang, Bing3 |
| 刊名 | ADVANCED FUNCTIONAL MATERIALS
 |
| 出版日期 | 2015-07-22 |
| 卷号 | 25期号:28页码:4412-4418 |
| 关键词 | calmodulin conformation changes cationic-conjugated polymers graphene oxide |
| 英文摘要 | The conformation of calmodulin (CaM) changes from closed configuration to open one, converting to a claviform dumbbell-shaped biomolecule upon Ca2+-binding. A hybrid probe of graphene oxide (GO) cationic conjugated polymer for detection of the conformation transition of CaM by using FRET technique is demonstrated. The stronger hydrophobic interaction and weaker electrostatic repulsion leads to more CaM adsorption to the surface of GO upon binding with Ca2+ than that of CaM in the absence of Ca2+ (apoCaM), resulting in much farther proximity between poly[(9,9-bis(6-N,N,N-trimethylammonium)hexyl)-fluorenylene phenylene dibromide] (PFP) and green fluorescent protein labeled at the N-terminus of CaM and therefore much weaker FRET efficiency for PFP/Ca2+/CaM in comparison with that of PFP/apoCaM in the presence of GO. Notably, the assembly of CaM with GO is quantitatively and reversibly controlled by Ca2+ ions. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/27772]  |
| 专题 | 化学研究所_有机固体实验室 |
| 作者单位 | 1.Hebei Univ Technol, Inst Biophys, Key Lab Hebei Prov Mol Biophys, Tianjin 300401, Peoples R China 2.Hebei Univ Technol, Sch Mat Sci & Engn, Tianjin 300130, Peoples R China 3.Chinese Acad Sci, Inst Chem, Key Lab Organ Solids, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yuan, Hongbo,Qi, Junjie,Xing, Chengfen,et al. Graphene-Oxide-Conjugated Polymer Hybrid Materials for Calmodulin Sensing by Using FRET Strategy[J]. ADVANCED FUNCTIONAL MATERIALS,2015,25(28):4412-4418. |
| APA | Yuan, Hongbo.,Qi, Junjie.,Xing, Chengfen.,An, Hailong.,Niu, Ruimin.,...&Wang, Shu.(2015).Graphene-Oxide-Conjugated Polymer Hybrid Materials for Calmodulin Sensing by Using FRET Strategy.ADVANCED FUNCTIONAL MATERIALS,25(28),4412-4418. |
| MLA | Yuan, Hongbo,et al."Graphene-Oxide-Conjugated Polymer Hybrid Materials for Calmodulin Sensing by Using FRET Strategy".ADVANCED FUNCTIONAL MATERIALS 25.28(2015):4412-4418. |
入库方式： OAI收割
来源：化学研究所
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