Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers
文献类型：期刊论文
| 作者 | Jiang, Chao1,2; Yuan, Yuan1; Liu, Libing3; Hou, Jingyi2; Jin, Yan1; Huang, Luqi1 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2015-11-05 |
| 卷号 | 5 |
| 英文摘要 | A label-free, homogenous and sensitive one-step method for the molecular authentication of medicinal snakes has been developed by combining a rapid PCR technique with water-soluble cationic conjugated polyelectrolytes (CCPs). Three medicinal snake materials (Deinagkistrodon acutus, Zaocys dhumnades and Bungarus multicinctus; a total of 35 specimens) and 48 snake specimens with similar morphologies and textures were clearly distinguished by the naked eye by utilizing a CCP-based assay in a high-throughput manner. The identification of medicinal snakes in patented Chinese drugs was successfully performed using this detection system. In contrast to previous fluorescence-labeled oligonucleotide detection and direct DNA stain hybridization assays, this method does not require designing dye-labeled primers, and unfavorable dimer fluorescence is avoided in this homogenous method. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/29177]  |
| 专题 | 化学研究所_有机固体实验室 |
| 作者单位 | 1.China Acad Chinese Med Sci, Natl Resources Ctr Chinese Mat Med, State Key Lab Breeding Base Dao Di Herbs, Beijing 100700, Peoples R China 2.Beijing Normal Univ, Coll Resources, Beijing Area Major Lab Protect & Utilizat Tradit, Beijing 100875, Peoples R China 3.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Inst Chem, Key Lab Organ Solids, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Chao,Yuan, Yuan,Liu, Libing,et al. Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers[J]. SCIENTIFIC REPORTS,2015,5. |
| APA | Jiang, Chao,Yuan, Yuan,Liu, Libing,Hou, Jingyi,Jin, Yan,&Huang, Luqi.(2015).Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers.SCIENTIFIC REPORTS,5. |
| MLA | Jiang, Chao,et al."Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers".SCIENTIFIC REPORTS 5(2015). |
入库方式： OAI收割
来源：化学研究所
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