Fluorescence Ratiometric Assay Strategy for Chemical Transmitter of Living Cells Using H2O2-Sensitive Conjugated Polymers
文献类型：期刊论文
| 作者 | Wang, Yunxia; Li, Shengliang; Feng, Liheng; Nie, Chenyao; Liu, Libing; Lv, Fengting; Wang, Shu |
| 刊名 | ACS Applied Materials & Interfaces
 |
| 出版日期 | 2015-11-04 |
| 卷号 | 7期号:43页码:24110-24118 |
| 关键词 | water-soluble conjugated polymers FRET detection chemical transmitter living cells |
| 英文摘要 | A new water-soluble conjugated poly(fluorene-co-phenylene) derivative (PFP-FB) modified with boronate-protected fluorescein (peroxyfluor-1) via PEG linker has been designed and synthesized. In the presence of H2O2, the peroxyfluor-1 group can transform into green fluorescent fluorescein by deprotecting the boronate protecting groups. In this case, upon selective excitation of PFP-FB backbone at 380 nm, efficient fluorescence resonance energy transfer (FRET) from PFP-FB backbone to fluorescein occurs, and accordingly, the fluorescence color of PFP-FB changes from blue to green. Furthermore, the emission color of PFP-FB and the FRET ratio change in a concentration-dependent manner. By taking advantage of PFP-FB, ratiometric detection of choline and acetylcholine (ACh) through cascade enzymatic reactions and further dynamic monitoring of the choline consumption process of cancer cells have been successfully realized. Thus, this new polymer probe promotes the development of enzymatic biosensors and provides a simpler and more effective way for detecting the chemical transmitter of living cells. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/29224]  |
| 专题 | 化学研究所_有机固体实验室 |
| 作者单位 | Chinese Acad Sci, Inst Chem, Key Lab Organ Solids, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yunxia,Li, Shengliang,Feng, Liheng,et al. Fluorescence Ratiometric Assay Strategy for Chemical Transmitter of Living Cells Using H2O2-Sensitive Conjugated Polymers[J]. ACS Applied Materials & Interfaces,2015,7(43):24110-24118. |
| APA | Wang, Yunxia.,Li, Shengliang.,Feng, Liheng.,Nie, Chenyao.,Liu, Libing.,...&Wang, Shu.(2015).Fluorescence Ratiometric Assay Strategy for Chemical Transmitter of Living Cells Using H2O2-Sensitive Conjugated Polymers.ACS Applied Materials & Interfaces,7(43),24110-24118. |
| MLA | Wang, Yunxia,et al."Fluorescence Ratiometric Assay Strategy for Chemical Transmitter of Living Cells Using H2O2-Sensitive Conjugated Polymers".ACS Applied Materials & Interfaces 7.43(2015):24110-24118. |
入库方式： OAI收割
来源：化学研究所
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