Visual Detection of Multiplex MicroRNAs Using Cationic Conjugated Polymer Materials
文献类型：期刊论文
| 作者 | Zhou, Yuanyuan1; Zhang, Jiangyan1; Zhao, Likun1; Li, Yingcun1; Chen, Hui2; Li, Shengliang2; Cheng, Yongqiang1 |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2016-01-20 |
| 卷号 | 8期号:2页码:1520-1526 |
| 关键词 | cationic conjugated polymer microRNA FRET multiplex detection visual detection |
| 英文摘要 | A simple, visual, and specific method for simultaneous detection of multiplex microRNAs (miRNAs) has been developed by integrating duplex-specific nuclease (DSN)-induced amplification with cationic conjugated polymer (CCP) materials. The probe DNA with a complementary sequence to target miRNA is labeled with fluorescein dye (FAM). Without target miRNA, the single-strand DNA probe cannot be digested by DSN. Upon adding CCPs, efficient fluorescence resonance energy transfer (FRET) from CCP to FAM occurs owing to strong electrostatic interactions between CCP and the DNA probe. In the presence of target miRNA, the DNA probe hybridizes with target miRNA followed by digestion to small nucleotide fragments by DSN; meanwhile, the miRNA is released and subsequently interacts again with the probe, resulting in the cycled digestion of the DNA probe. In this case, weak electrostatic interactions between oligonucleotide fragments and CCP lead to inefficient FRET from CCP to FAM. Thus, by triggering the FRET signal from CCP to FAM, miRNA can be specially detected, and the fluorescence color change based on FRET can be visualized directly with the naked eye under an UV lamp. Furthermore, an energy transfer cascade can be designed using CCP and DNA probes labeled at the 5'-terminus with FAM and Cy3 dyes, and the multistep FRET processes offer the ability of simultaneous detection of multiplex miRNAs. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/30069]  |
| 专题 | 化学研究所_有机固体实验室 |
| 作者单位 | 1.Hebei Univ, Coll Chem & Environm Sci, Key Lab Analyt Sci & Technol Hebei Prov, Minist Educ,Key Lab Med Chem & Mol Diag, Baoding 071002, Hebei, Peoples R China 2.Chinese Acad Sci, Inst Chem, Key Lab Organ Solids, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Yuanyuan,Zhang, Jiangyan,Zhao, Likun,et al. Visual Detection of Multiplex MicroRNAs Using Cationic Conjugated Polymer Materials[J]. ACS APPLIED MATERIALS & INTERFACES,2016,8(2):1520-1526. |
| APA | Zhou, Yuanyuan.,Zhang, Jiangyan.,Zhao, Likun.,Li, Yingcun.,Chen, Hui.,...&Cheng, Yongqiang.(2016).Visual Detection of Multiplex MicroRNAs Using Cationic Conjugated Polymer Materials.ACS APPLIED MATERIALS & INTERFACES,8(2),1520-1526. |
| MLA | Zhou, Yuanyuan,et al."Visual Detection of Multiplex MicroRNAs Using Cationic Conjugated Polymer Materials".ACS APPLIED MATERIALS & INTERFACES 8.2(2016):1520-1526. |
入库方式： OAI收割
来源：化学研究所
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