A Novel Electrochemiluminescence Immunosensor for the Analysis of HIV-1 p24 Antigen Based on P-RGO@Au@Ru-SiO2 Composite
文献类型：期刊论文
| 作者 | Zhou,Limin; Huang,Jianshe; Yu,Bin; Liu,Yang; You,Tianyan |
| 刊名 | acs applied materials & interfaces
 |
| 出版日期 | 2015 |
| 卷号 | 7期号:44页码:24438-24445 |
| 关键词 | RESONANCE ENERGY-TRANSFER NITROGEN-DOPED GRAPHENE SENSITIVE DNA DETECTION ULTRASENSITIVE DETECTION SILICA NANOPARTICLES SIGNAL AMPLIFICATION MODIFIED ELECTRODE ENHANCED ELECTROCHEMILUMINESCENCE ELECTROCHEMICAL IMMUNOSENSOR VIRUS-ANTIBODY |
| 通讯作者 | you,ty |
| 英文摘要 | ru(bpy)(3)(2+)-doped silica (ru-sio2) nanoparticles and gold-nanoparticle-decorated graphene (p-rgo@au) were combined to form a p-rgo@au@ru-sio2 composite. the composite was used to develop a novel sandwich-type electrochemiluminescence immunosensor for the analysis of hiv-1 p24 antigen. the composite worked as carrier to immobilize target antibody and to build a sandwich-type electrochemiluminescence immunosensor through an interaction between antigen and antibody. importantly, high ecl signal could be obtained due to the large amounts of ru(bpy)(3)(2+) molecules per ru-sio2 nanopartide. the p-rgo@au composite with good conductivity and high surface area not only accelerated the electron transfer rate but also improved the loading of both ecl molecules and capture antibody, which could further increase the ecl response and result in high sensitivity. taking advantage of both ru-sio2 nanoparticles and the p-rgo@au composite, the proposed immunosensor exhibited a linear range from 1.0 x 10(-9) to 1.0 x 10(-5) mg ml(-1) with a detection limit of 1.0 x 10(-9) mg ml(-1) for hiv-1 p24 antigen. the proposed ecl immunosensor was used to analyze hiv-1 p24 antigen in human serum, and satisfactory recoveries were obtained, indicating that the proposed method is promising for practical applications in the clinical diagnosis of hiv infection. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000364726400008 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/64756]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhou,Limin,Huang,Jianshe,Yu,Bin,et al. A Novel Electrochemiluminescence Immunosensor for the Analysis of HIV-1 p24 Antigen Based on P-RGO@Au@Ru-SiO2 Composite[J]. acs applied materials & interfaces,2015,7(44):24438-24445. |
| APA | Zhou,Limin,Huang,Jianshe,Yu,Bin,Liu,Yang,&You,Tianyan.(2015).A Novel Electrochemiluminescence Immunosensor for the Analysis of HIV-1 p24 Antigen Based on P-RGO@Au@Ru-SiO2 Composite.acs applied materials & interfaces,7(44),24438-24445. |
| MLA | Zhou,Limin,et al."A Novel Electrochemiluminescence Immunosensor for the Analysis of HIV-1 p24 Antigen Based on P-RGO@Au@Ru-SiO2 Composite".acs applied materials & interfaces 7.44(2015):24438-24445. |
入库方式： OAI收割
来源：长春应用化学研究所
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