Highly sensitive and selective colorimetric detection of glutathione based on Ag [I] ion-3,3 ',5,5 '-tetramethylbenzidine (TMB)
文献类型：期刊论文
| 作者 | Ni,Pengjuan; Sun,Yujing; Dai,Haichao; Hu,Jingting; Jiang,Shu; Wang,Yilin; Li,Zhuang |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2015 |
| 卷号 | 63期号:2页码:47-52 |
| 关键词 | GOLD NANOPARTICLES LABEL-FREE PEROXIDASE MIMETICS REDUCED GLUTATHIONE MASS-SPECTROMETRY QUANTUM DOTS G-QUADRUPLEX BIOTHIOLS THIOLS CELLS |
| 通讯作者 | li,z |
| 英文摘要 | glutathione (gsh) plays an important role in the biological system and serves many cellular functions. since all of the biothiols possess similar functional groups, it is still challenging to selectively detect gsh over cysteine (cys) and homocysteine (hcy). in this work, a novel and simple colorimetric method for discriminative detection of glutathione (gsh) over cys and hcy is developed. the proposed method is based on the fact that ag [i] ion could oxidize 3,3',5,5',-tetramethylbenzidine (tmb) to the oxidized tmb to induce a blue color and an absorption peak centered at 652 nm. however, the introduction of gsh could cause the reduction of oxidized tmb and it could also combine with ag+, both of which result in a blue color fading and a decrease of the absorbance at 652 nm. based on this finding, we propose a method to qualitatively and quantitatively detect gsh by naked eyes and uv-vis spectroscopy, respectively. the proposed method shows a low detection limit of 0.1 mu m by naked eyes and 0.05 mu m with the help of uv-vis spectroscopy. in addition, this method has great potential in discriminatively detecting gsh over other amino acid and biothiols. more importantly, this method is simple and fast without the preparation of nanomaterials and has also been successfully applied to the detection of gsh in biological fluids. (c) 2014 elsevier b.v. all rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/64763]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Ni,Pengjuan,Sun,Yujing,Dai,Haichao,et al. Highly sensitive and selective colorimetric detection of glutathione based on Ag [I] ion-3,3 ',5,5 '-tetramethylbenzidine (TMB)[J]. biosensors & bioelectronics,2015,63(2):47-52. |
| APA | Ni,Pengjuan.,Sun,Yujing.,Dai,Haichao.,Hu,Jingting.,Jiang,Shu.,...&Li,Zhuang.(2015).Highly sensitive and selective colorimetric detection of glutathione based on Ag [I] ion-3,3 ',5,5 '-tetramethylbenzidine (TMB).biosensors & bioelectronics,63(2),47-52. |
| MLA | Ni,Pengjuan,et al."Highly sensitive and selective colorimetric detection of glutathione based on Ag [I] ion-3,3 ',5,5 '-tetramethylbenzidine (TMB)".biosensors & bioelectronics 63.2(2015):47-52. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。