Highly sensitive and specific colorimetric detection of cancer cells via dual-aptamer target binding strategy
文献类型：期刊论文
| 作者 | Wang,Kun; Fan,Daoqing; Liu,Yaqing; Wang,Erkang |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2015 |
| 卷号 | 73页码:1-6 |
| 关键词 | ENDOTHELIAL GROWTH-FACTOR NANOPARTICLE AMPLIFICATION GOLD NANOPARTICLES BREAST PROTEIN ASSAY FUNCTIONALIZATION RECOGNITION APTASENSOR MARKERS |
| 通讯作者 | wang,ek |
| 英文摘要 | simple, rapid, sensitive and specific detection of cancer cells is of great importance for early and accurate cancer diagnostics and therapy. by coupling nanotechnology and dual-aptamer target binding strategies, we developed a colorimetric assay for visually detecting cancer cells with high sensitivity and specificity. the nanotechnology including high catalytic activity of ptaunp and magnetic separation & concentration plays a vital role on the signal amplification and improvement of detection sensitivity. the color change caused by small amount of target cancer cells (10 cells/ml) can be clearly distinguished by naked eyes. the dual-aptamer target binding strategy guarantees the detection specificity that large amount of non-cancer cells and different cancer cells (10(4) cells/ml) cannot cause obvious color change. a detection limit as low as 10 cells/ml with detection linear range from 10 to 10(5) cells/ml was reached according to the experimental detections in phosphate buffer solution as well as serum sample. the developed enzymefree and cost effective calorimetric assay is simple and no need of instrument while still provides excellent sensitivity, specificity and repeatability, having potential application on point-of-care cancer diagnosis. (c) 2015 elsevier b.v. all rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000358823900001 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/64767]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang,Kun,Fan,Daoqing,Liu,Yaqing,et al. Highly sensitive and specific colorimetric detection of cancer cells via dual-aptamer target binding strategy[J]. biosensors & bioelectronics,2015,73:1-6. |
| APA | Wang,Kun,Fan,Daoqing,Liu,Yaqing,&Wang,Erkang.(2015).Highly sensitive and specific colorimetric detection of cancer cells via dual-aptamer target binding strategy.biosensors & bioelectronics,73,1-6. |
| MLA | Wang,Kun,et al."Highly sensitive and specific colorimetric detection of cancer cells via dual-aptamer target binding strategy".biosensors & bioelectronics 73(2015):1-6. |
入库方式： OAI收割
来源：长春应用化学研究所
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