A label-free fluorescent molecular beacon based on DNA-Ag nanoclusters for the construction of versatile Biosensors
文献类型：期刊论文
| 作者 | Cao,Qiao; Teng,Ye; Yang,Xuan; Wang,Jin; Wang,Erkang |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2015 |
| 卷号 | 74页码:318-321 |
| 关键词 | SILVER NANOCLUSTERS LIVING CELLS METAL NANOCLUSTERS LOGIC DEVICES HYBRIDIZATION PROBE IMMOBILIZATION FLUOROPHORES CHALLENGES CLUSTERS |
| 通讯作者 | wang,e |
| 英文摘要 | in this paper, we developed a simple, low-cost and sensitive dna sequences detection biosensor based on a label-free molecular beacon (mb) whose dna hairpin structure terminal has a guanine-rich sequence that can enhance fluorescence of silver nanoclusters (ag ncs). without hybridization between hairpin probe and target dna, the ag ncs presented bright fluorescence for the proximity of guanine-rich sequences (grss). after binding with target dna, the hairpin shape was destroyed which results in a decrease of the ag ncs fluorescence intensity. with this biosensor, we detected three disease-related genes that were the human immunodeficiency virus (hiv) gene, hepatitis b virus (hbv) gene and human t-lymphotropic virus type i (htlv-i) gene. the detection limits based on s/n of 3 were 4.4 nm, 6.8 nm and 8.5 nm for hiv gene, hbv gene and htlv-i gene, respectively. our sensor was also of high selectivity and could distinguish even one nucleotide mismatched target. (c) 2015 elsevier b.v. all rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000360772800042 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/64770]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Cao,Qiao,Teng,Ye,Yang,Xuan,et al. A label-free fluorescent molecular beacon based on DNA-Ag nanoclusters for the construction of versatile Biosensors[J]. biosensors & bioelectronics,2015,74:318-321. |
| APA | Cao,Qiao,Teng,Ye,Yang,Xuan,Wang,Jin,&Wang,Erkang.(2015).A label-free fluorescent molecular beacon based on DNA-Ag nanoclusters for the construction of versatile Biosensors.biosensors & bioelectronics,74,318-321. |
| MLA | Cao,Qiao,et al."A label-free fluorescent molecular beacon based on DNA-Ag nanoclusters for the construction of versatile Biosensors".biosensors & bioelectronics 74(2015):318-321. |
入库方式： OAI收割
来源：长春应用化学研究所
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