Chemiluminescence Detection of a Protein through the Aptamer-Controlled Catalysis of a Porphyrin Probe
文献类型：期刊论文
| 作者 | Li,Wenying; Zhang; Qingfeng; Zhou,Huipeng; Chen,Jian; Li,Yongxin; Zhang,Cuiyun; Yu,Cong |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2015 |
| 卷号 | 87期号:16页码:8336-8341 |
| 关键词 | ENDOTHELIAL GROWTH-FACTOR TURN-ON DETECTION PERYLENE PROBE FACTOR VEGF TUMOR ANGIOGENESIS AQUEOUS-SOLUTIONS DNA APTAMER AGGREGATION FLUORESCENCE APTASENSOR |
| 通讯作者 | yu,c |
| 英文摘要 | sensitive and selective protein detection based on the aptamer-controlled noncovalent porphyrin probe self-assembly is reported for the first time. vascular endothelial growth factor (vegf) is a predominant biomarker in cancer angiogenesis. in this work, a positively charged porphyrin probe, manganese(iii) meso-tetrakis(n-methylpyridinum-4-yl)porphyrin (mn-pyp), was prepared. using it as a catalyst, a label-free chemiluminescence (cl) turn-on approach for sensitive vegf detection is developed. mn-pyp could catalyze the luminol cl reaction. the vegf aptamer could induce aggregation of mn-pyp. as a result, the mn-pyp-catalyzed cl reaction is efficiently suppressed. upon the addition of vegf, the specific binding of vegf to the aptamer weakens the interactions between the aptarner and mn-pyp. the mn-pyp monomers are released, and a turn-on cl signal is thus detected. our method is quite sensitive; so pm of vegf could be easily detected. it is also very selective against other proteins. our assay provides an aptamer-based efficient way for protein quantification. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000359892100044 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/64841]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li,Wenying,Zhang,Qingfeng,et al. Chemiluminescence Detection of a Protein through the Aptamer-Controlled Catalysis of a Porphyrin Probe[J]. analytical chemistry,2015,87(16):8336-8341. |
| APA | Li,Wenying.,Zhang.,Qingfeng.,Zhou,Huipeng.,Chen,Jian.,...&Yu,Cong.(2015).Chemiluminescence Detection of a Protein through the Aptamer-Controlled Catalysis of a Porphyrin Probe.analytical chemistry,87(16),8336-8341. |
| MLA | Li,Wenying,et al."Chemiluminescence Detection of a Protein through the Aptamer-Controlled Catalysis of a Porphyrin Probe".analytical chemistry 87.16(2015):8336-8341. |
入库方式： OAI收割
来源：长春应用化学研究所
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