Methanol-Promoted Lipid Remodelling during Cooling Sustains Cryopreservation Survival of Chlamydomonas reinhardtii
文献类型：期刊论文
| 作者 | Yang, Duanpeng; Li, Weiqi
|
| 刊名 | PLOS ONE
 |
| 出版日期 | 2016-01-05 |
| 卷号 | 11期号:1 |
| 英文摘要 | Cryogenic treatments and cryoprotective agents (CPAs) determine the survival rate of organisms that undergo cryopreservation, but their mechanisms of operation have not yet been characterised adequately. In particular, the way in which membrane lipids respond to cryogenic treatments and CPAs is unknown. We developed comparative profiles of the changes in membrane lipids among cryogenic treatments and between the CPAs dimethyl sulfoxide (DMSO) and methanol (MeOH) for the green alga Chlamydomonas reinhardtii. We found that freezing in liquid nitrogen led to a dramatic degradation of lipids, and that thawing at warm temperature (35 degrees C) induced lipid remodelling. DMSO did not protect membranes, but MeOH significantly attenuated lipid degradation. The presence of MeOH during cooling (from 25 degrees C to -55 degrees C at a rate of 1 degrees C/min) sustained the lipid composition to the extent that membrane integrity was maintained; this phenomenon accounts for successful cryopreservation. An increase in monogalactosyldiacylglycerol and a decrease in diacylglycerol were the major changes in lipid composition associated with survival rate, but there was no transformation between these lipid classes. Phospholipase D-mediated phosphatidic acid was not involved in freezing-induced lipid metabolism in C. reinhardtii. Lipid unsaturation changed, and the patterns of change depended on the cryogenic treatment. Our results provide new insights into the cryopreservation of, and the lipid metabolism in, algae. |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | PHOSPHOLIPASE-D ; DROPLET-VITRIFICATION ; ARABIDOPSIS-THALIANA ; MEMBRANE-LIPIDS ; GREEN-ALGAE ; STRESS ; TOLERANCE ; TRIACYLGLYCEROL ; BIOSYNTHESIS ; DEGRADATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000367801400130 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/25451]  |
| 专题 | 昆明植物研究所_中国西南野生生物种质资源库 |
| 推荐引用方式 GB/T 7714 | Yang, Duanpeng,Li, Weiqi. Methanol-Promoted Lipid Remodelling during Cooling Sustains Cryopreservation Survival of Chlamydomonas reinhardtii[J]. PLOS ONE,2016,11(1). |
| APA | Yang, Duanpeng,&Li, Weiqi.(2016).Methanol-Promoted Lipid Remodelling during Cooling Sustains Cryopreservation Survival of Chlamydomonas reinhardtii.PLOS ONE,11(1). |
| MLA | Yang, Duanpeng,et al."Methanol-Promoted Lipid Remodelling during Cooling Sustains Cryopreservation Survival of Chlamydomonas reinhardtii".PLOS ONE 11.1(2016). |
入库方式： OAI收割
来源：昆明植物研究所
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