Identification and characterization of an insect toxin protein, Bb70p, from the entomopathogenic fungus, Beauveria bassiana, using Galleria mellonella as a model system
文献类型：期刊论文
| 作者 | Khan, Sehroon; Nadir, Sadia; Guo Lihua; Xu, Jianchu ; Holmes, Keith A.; Qiu Dewen
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| 刊名 | JOURNAL OF INVERTEBRATE PATHOLOGY
 |
| 出版日期 | 2016 |
| 卷号 | 133页码:87-94 |
| 关键词 | Insect-toxic protein Bb70p Entomopathogenic fungi Beauveria bassiana Galleria mellonella Filtrate |
| 英文摘要 | An insect-toxic protein, Bb70p, was purified from Beauveria bassiana 70 using ammonium sulfate precipitation, ion exchange chromatography, and gel filtration. Bb70p has a high affinity for anion exchangers and 2D electrophoresis results revealed a single spot with a molecular weight of 35.5 kDa and an iso-electric point of similar to 4.5. Bb70p remains active from 4 to 60 degrees C, within a pH range of 4-10, but is more active in slightly acidic pH. A pure protein, Bb70p does not have any carbohydrate side chains. The protein caused high mortality by intra-haemocelic injection into Galleria mellonella with LD50 of 334.41 mu g/g body weight and activates the phenol oxidase cascade. With a partial amino acid sequence comparison using the NCBI database, we showed no homology to known toxin proteins of entomopathogenic fungi. Thus, Bb70p appears to be an insect toxin protein, demonstrating novelty. Identification of this insect-toxic protein presents potential to enhance the virulence of B. bassiana through genetic manipulation. (C) 2015 Elsevier Inc. All rights reserved. |
| 类目[WOS] | Zoology |
| 研究领域[WOS] | Zoology |
| 关键词[WOS] | MOSQUITO LARVAE ; IN-VITRO ; HOST ; HEMOLYMPH ; VIRULENCE ; BIOPESTICIDES ; PATHOGENS ; PROTEASES ; STRAINS ; VIVO |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000368317200013 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/25448]  |
| 专题 | 昆明植物研究所_资源植物与生物技术所级重点实验室 |
| 推荐引用方式 GB/T 7714 | Khan, Sehroon,Nadir, Sadia,Guo Lihua,et al. Identification and characterization of an insect toxin protein, Bb70p, from the entomopathogenic fungus, Beauveria bassiana, using Galleria mellonella as a model system[J]. JOURNAL OF INVERTEBRATE PATHOLOGY,2016,133:87-94. |
| APA | Khan, Sehroon,Nadir, Sadia,Guo Lihua,Xu, Jianchu,Holmes, Keith A.,&Qiu Dewen.(2016).Identification and characterization of an insect toxin protein, Bb70p, from the entomopathogenic fungus, Beauveria bassiana, using Galleria mellonella as a model system.JOURNAL OF INVERTEBRATE PATHOLOGY,133,87-94. |
| MLA | Khan, Sehroon,et al."Identification and characterization of an insect toxin protein, Bb70p, from the entomopathogenic fungus, Beauveria bassiana, using Galleria mellonella as a model system".JOURNAL OF INVERTEBRATE PATHOLOGY 133(2016):87-94. |
入库方式： OAI收割
来源：昆明植物研究所
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