Sample Preparation and Stability of Human Serum and Urine Based on HPLC-DAD for Metabonomics Studies
文献类型：期刊论文
| 作者 | Liu Y(刘芸)1,2,3 ; Sun XM(孙小明)1,2,3; Di DL(邸多隆)1,2; Feng, Yuxiang4; Jin, Fengling5
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| 刊名 | Bulletin- Korean Chemical Society
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| 出版日期 | 2012 |
| 卷号 | 33期号:7页码:2156-2162 |
| 关键词 | Stability HPLC/UPLC Serum Urine Metabonomics |
| ISSN号 | 0253-2964 |
| 英文摘要 | Many literatures focus on the biological relevance and the identification of biomarkers for disease activity assessment while less attention has been paid to the development of standard procedures for sample preparation and storage based on liquid chromatography technique. The influencing factors including protein precipitation, storage temperature, storage time, and reconstitution by ultra pure water were analyzed employing HPLC-DAD. The effects were investigated from five participants over three months by principal components analysis (PCA) and the values of percent changes (PC). The samples with protein precipitation might slow the rate of bacterial enzymatic conversion. After protein precipitation, the average PC of urine samples (0.136 ± 0.013, n = 5) is relatively less than that of the serum samples (0.173 ± 0.026, n = 5) for three months. Minimal effects on metabolic profiles of serum and urine (PC < 0.15) are reasonable for metabolomic studies after protein precipitation and storage at -20 ℃ for two months. |
| 学科主题 | 分析化学与药物化学 |
| 收录类别 | SCI |
| 资助信息 | the "Hundred Talents Program" of Chinese Academy Sciences (CAS) in 2007;the Natiomal Natural Science Foundation of China (NSFC No. 20775083) |
| 语种 | 英语 |
| WOS记录号 | WOS:000306818100008 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://210.77.64.217/handle/362003/19563]  |
| 专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem NW Plant Resources, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 4.Gansu Prov Hosp Tradit Chinese Med, Lanzhou 730000, Peoples R China 5.Lanzhou Univ, Affiliated Hosp 1, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu Y,Sun XM,Di DL,et al. Sample Preparation and Stability of Human Serum and Urine Based on HPLC-DAD for Metabonomics Studies[J]. Bulletin- Korean Chemical Society,2012,33(7):2156-2162. |
| APA | Liu Y,Sun XM,Di DL,Feng, Yuxiang,&Jin, Fengling.(2012).Sample Preparation and Stability of Human Serum and Urine Based on HPLC-DAD for Metabonomics Studies.Bulletin- Korean Chemical Society,33(7),2156-2162. |
| MLA | Liu Y,et al."Sample Preparation and Stability of Human Serum and Urine Based on HPLC-DAD for Metabonomics Studies".Bulletin- Korean Chemical Society 33.7(2012):2156-2162. |
入库方式： OAI收割
来源：兰州化学物理研究所
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