中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Sample Preparation and Stability of Human Serum and Urine Based on HPLC-DAD for Metabonomics Studies

文献类型:期刊论文

作者Liu Y(刘芸)1,2,3; Sun XM(孙小明)1,2,3; Di DL(邸多隆)1,2; Feng, Yuxiang4; Jin, Fengling5
刊名Bulletin- Korean Chemical Society
出版日期2012
卷号33期号:7页码:2156-2162
关键词Stability HPLC/UPLC Serum Urine Metabonomics
ISSN号0253-2964
英文摘要Many literatures focus on the biological relevance and the identification of biomarkers for disease activity assessment while less attention has been paid to the development of standard procedures for sample preparation and storage based on liquid chromatography technique. The influencing factors including protein precipitation, storage temperature, storage time, and reconstitution by ultra pure water were analyzed employing HPLC-DAD. The effects were investigated from five participants over three months by principal components analysis (PCA) and the values of percent changes (PC). The samples with protein precipitation might slow the rate of bacterial enzymatic conversion. After protein precipitation, the average PC of urine samples (0.136 ± 0.013, n = 5) is relatively less than that of the serum samples (0.173 ± 0.026, n = 5) for three months. Minimal effects on metabolic profiles of serum and urine (PC < 0.15) are reasonable for metabolomic studies after protein precipitation and storage at -20 ℃ for two months.
学科主题分析化学与药物化学
收录类别SCI
资助信息the "Hundred Talents Program" of Chinese Academy Sciences (CAS) in 2007;the Natiomal Natural Science Foundation of China (NSFC No. 20775083)
语种英语
WOS记录号WOS:000306818100008
公开日期2016-05-09
源URL[http://210.77.64.217/handle/362003/19563]  
专题兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室
作者单位1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem NW Plant Resources, Lanzhou 730000, Peoples R China
2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
4.Gansu Prov Hosp Tradit Chinese Med, Lanzhou 730000, Peoples R China
5.Lanzhou Univ, Affiliated Hosp 1, Lanzhou 730000, Peoples R China
推荐引用方式
GB/T 7714
Liu Y,Sun XM,Di DL,et al. Sample Preparation and Stability of Human Serum and Urine Based on HPLC-DAD for Metabonomics Studies[J]. Bulletin- Korean Chemical Society,2012,33(7):2156-2162.
APA Liu Y,Sun XM,Di DL,Feng, Yuxiang,&Jin, Fengling.(2012).Sample Preparation and Stability of Human Serum and Urine Based on HPLC-DAD for Metabonomics Studies.Bulletin- Korean Chemical Society,33(7),2156-2162.
MLA Liu Y,et al."Sample Preparation and Stability of Human Serum and Urine Based on HPLC-DAD for Metabonomics Studies".Bulletin- Korean Chemical Society 33.7(2012):2156-2162.

入库方式: OAI收割

来源:兰州化学物理研究所

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