Separation of abietane-type diterpenoids from Clerodendrum kaichianum Hsu by high-speed counter-current chromatography using stepwise elution
文献类型：期刊论文
| 作者 | Xu, Mingfeng ; Zhu, Qin ; Wang, Huizhong ; Du, Qizhen ; Xu, Maojun |
| 刊名 | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
 |
| 出版日期 | 2016 |
| 卷号 | 39期号:3页码:139-144 |
| 关键词 | PETASITES S. MOORE PURIFICATION ROOT MANDARINORUM DELIVERY GROWTH BUNGEI ASSAY |
| ISSN号 | 1082-6076 |
| 中文摘要 | High-speed counter-current chromatography (HSCCC) was successfully used for the separation of abietane-type diterpenoids from the medicinal plant C. kaichianum, which were not separated in our previous study using preparative HPLC. The HSCCC separation employed the lower phases of n-hexane-ethyl acetate-methanol-water (HEMW) 4:5:4:5 and HEMW 4:5:5:4 as the mobile phase for stepwise elution while the upper phase of HEMW 4:5:4:5 was used as the stationary phase. HSCCC separation yielded 90.5mg of compound 1(kaichianone A), 137.7mg of compound 2 (kaichianone B), 125.0mg of compound 3 (teuvincenone E), and 227.6mg of compound 4 (taxusabietane A) with purities of 95.3%, 97.2%, 97.8%, and 98.6%, respectively, as determined by HPLC. Compounds 1-2 are two new abietane-type diterpenoids while Compounds 3-4 are known abietane-type diterpenoids, analyzed by ESIMS and NMR data. The results demonstrated that HSCCC can be an excellent alternative for other separation methods. The two new compounds showed significant cytotoxicity against ileocecal carcinoma HCT-8 and breast adenocarcinoma MCF-7 cells. |
| 学科主题 | Biochemistry & Molecular Biology ; Chemistry |
| 收录类别 | SCI |
| 原文出处 | 10.1080/10826076.2015.1137003 |
| 公开日期 | 2016-05-06 |
| 源URL | [http://ir.xtbg.org.cn/handle/353005/9743]  |
| 专题 | 西双版纳热带植物园_文献共享 |
| 推荐引用方式 GB/T 7714 | Xu, Mingfeng,Zhu, Qin,Wang, Huizhong,et al. Separation of abietane-type diterpenoids from Clerodendrum kaichianum Hsu by high-speed counter-current chromatography using stepwise elution[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2016,39(3):139-144. |
| APA | Xu, Mingfeng,Zhu, Qin,Wang, Huizhong,Du, Qizhen,&Xu, Maojun.(2016).Separation of abietane-type diterpenoids from Clerodendrum kaichianum Hsu by high-speed counter-current chromatography using stepwise elution.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,39(3),139-144. |
| MLA | Xu, Mingfeng,et al."Separation of abietane-type diterpenoids from Clerodendrum kaichianum Hsu by high-speed counter-current chromatography using stepwise elution".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 39.3(2016):139-144. |
入库方式： OAI收割
来源：西双版纳热带植物园
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