Facile Synthesis of Guanidyl-Functionalized Magnetic Polymer Microspheres for Tunable and Specific Capture of Global Phosphopeptides or Only Multiphosphopeptides
文献类型：期刊论文
| 作者 | Xiong, Zhichao1; Chen, Yajing1; Zhang, Lingyi1; Ren, Jun1; Zhang, Quanqing2; Ye, Mingliang2; Zhang, Weibing1; Zou, Hanfa2 |
| 刊名 | acs applied materials & interfaces
 |
| 出版日期 | 2014-12-24 |
| 卷号 | 6期号:24页码:22743-22750 |
| 关键词 | magnetic microspheres guanidyl tunable enrichment ability phosphorylated peptides mass spectrum |
| 通讯作者 | 张维冰 ; 邹汉法 |
| 英文摘要 | the highly selective and efficient capture of heterogeneous types of phosphopeptides is critical for comprehensive and in-depth phosphoproteome analysis, but it still remains a challenge since the lack of affinity material with large binding capacity and controllable specificity. here, a new affinity material was prepared to improve the enrichment capacity and endue the tunable specificity by introducing guanidyl onto poly(glycidyl methacrylate) (pgma) modified fe3o4 microsphere (denoted as fe3o4@pgma-guanidyl). the thick polymer shell endows the composite microsphere with large amount of guanidyl and is beneficial to enhancing the affinity interaction between phosphopeptides and the material. interestingly, the fe3o4@pgma-guanidyl possesses tunable enriching ability for global phosphopeptides or only multiphosphopeptides through simple regulation of buffer composition. the composite has large enrichment capacity (200 mg g(-1)), extremely high detection sensitivity (0.5 fmol), high enrichment recovery (91.30%), great specificity, and rapid magnetic separation. moreover, the result of the application to capture of phosphopeptides from tryptic digest of nonfat milk has demonstrated the great potential of fe3o4@pgma-guanidyl in detection and identification of low-abundance phosphopeptides of interest in biological sample. |
| WOS标题词 | science & technology ; technology |
| 学科主题 | 物理化学 |
| 类目[WOS] | nanoscience & nanotechnology ; materials science, multidisciplinary |
| 研究领域[WOS] | science & technology - other topics ; materials science |
| 关键词[WOS] | highly selective enrichment ; phosphorylated peptides ; rapid separation ; affinity probe ; iron-oxide ; nanoparticles ; shell ; immobilization ; glycopeptides ; composites |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000347139400120 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/143783]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.E China Univ Sci & Technol, Dept Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiong, Zhichao,Chen, Yajing,Zhang, Lingyi,et al. Facile Synthesis of Guanidyl-Functionalized Magnetic Polymer Microspheres for Tunable and Specific Capture of Global Phosphopeptides or Only Multiphosphopeptides[J]. acs applied materials & interfaces,2014,6(24):22743-22750. |
| APA | Xiong, Zhichao.,Chen, Yajing.,Zhang, Lingyi.,Ren, Jun.,Zhang, Quanqing.,...&Zou, Hanfa.(2014).Facile Synthesis of Guanidyl-Functionalized Magnetic Polymer Microspheres for Tunable and Specific Capture of Global Phosphopeptides or Only Multiphosphopeptides.acs applied materials & interfaces,6(24),22743-22750. |
| MLA | Xiong, Zhichao,et al."Facile Synthesis of Guanidyl-Functionalized Magnetic Polymer Microspheres for Tunable and Specific Capture of Global Phosphopeptides or Only Multiphosphopeptides".acs applied materials & interfaces 6.24(2014):22743-22750. |
入库方式： OAI收割
来源：大连化学物理研究所
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