Regulation of cell migration and osteogenic differentiation in mesenchymal stem cells under extremely low fluidic shear stress
文献类型：期刊论文
| 作者 | Gao, Xinghua1,2; Zhang, Xu1; Xu, Hui1; Zhou, Bingpu3; Wen, Weijia2,3; Qin, Jianhua1 |
| 刊名 | biomicrofluidics
 |
| 出版日期 | 2014-09-01 |
| 卷号 | 8期号:5页码:052008 |
| 通讯作者 | 秦建华 |
| 英文摘要 | mesenchymal stem cells (mscs) are multipotent stem cells predominantly obtained from bone marrow, which are sensitive to mechanical loadings in physiological microenvironment. however, how the mscs sense and respond to extremely low fluidic shear stress analogous to interstitial flow in vivo is poorly understood. in this work, we present a functional microfluidic device to examine the migration and differentiation behaviors of mscs in response to multiple orders of physiologically relevant interstitial flow levels. the different magnitudes of fluid flow-induced shear stress were produced by a hydraulic resistance-based microfluidic perfusion system consisting of a microchannel network and a parallel of uniform cell culture chambers. by changing the length and width of the flow-in channels, the multiple magnitudes of low shear stresses could be generated ranging from similar to 10(-5) to similar to 10(-2) dyne/cm(2). we demonstrated enhanced significant f-actin expression and cell migration in mscs under applied fluidic shear stress at similar to 10(-2) dyne/cm(2). we also demonstrated a significant osteogenic differentiation under this interstitial level of slow flows from similar to 10(-2) to similar to 10(-4) dyne/cm(2) in mscs by analyzing alkaline phosphatase activity and osteopontin staining. moreover, cytochalasin d and rho-inhibitor y-27632 significantly reduced the cytoskeleton f-actin expression and osteogenic differentiation in mscs, indicating the mediated mechanical responses of mscs under extremely low fluidic shear stress, possibly as a consequence of rho-associated kinase pathway. the established microfluidic perfusion system with multiple shear-flow capabilities is simple and easy to operate, providing a flexible platform for studying the responses of diverse types of cells to the multiple interstitial flow levels in a single assay. (c) 2014 aip publishing llc. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | biochemical research methods ; biophysics ; nanoscience & nanotechnology ; physics, fluids & plasmas |
| 研究领域[WOS] | biochemistry & molecular biology ; biophysics ; science & technology - other topics ; physics |
| 关键词[WOS] | bone-marrow ; signaling pathways ; dynamic culture ; flow ; microfluidics ; proliferation ; bioreactors |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000344226200010 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/143946]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Hong Kong Univ Sci & Technol, Med Ctr, Shenzhen Peking Univ, Biomed Res Inst, Shenzhen 518000, Peoples R China 3.Hong Kong Univ Sci & Technol, Micro Nanofluid Joint Lab, Kowloon, Hong Kong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Xinghua,Zhang, Xu,Xu, Hui,et al. Regulation of cell migration and osteogenic differentiation in mesenchymal stem cells under extremely low fluidic shear stress[J]. biomicrofluidics,2014,8(5):052008. |
| APA | Gao, Xinghua,Zhang, Xu,Xu, Hui,Zhou, Bingpu,Wen, Weijia,&Qin, Jianhua.(2014).Regulation of cell migration and osteogenic differentiation in mesenchymal stem cells under extremely low fluidic shear stress.biomicrofluidics,8(5),052008. |
| MLA | Gao, Xinghua,et al."Regulation of cell migration and osteogenic differentiation in mesenchymal stem cells under extremely low fluidic shear stress".biomicrofluidics 8.5(2014):052008. |
入库方式： OAI收割
来源：大连化学物理研究所
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