Direct Reprogramming of Human Fibroblasts to Functional and Expandable Hepatocytes
文献类型：期刊论文
| 作者 | Huang, Pengyu1; Zhang, Ludi1; Gao, Yimeng1; He, Zhiying2; Yao, Dan3; Wu, Zhitao3; Cen, Jin1; Chen, Xiaotao1; Liu, Changcheng2; Hu, Yiping2 |
| 刊名 | cell stem cell
 |
| 出版日期 | 2014-03-06 |
| 卷号 | 14期号:3页码:370-384 |
| 通讯作者 | lijianhui |
| 英文摘要 | the generation of large numbers of functional human hepatocytes for cell-based approaches to liver disease is an important and unmet goal. direct reprogramming of fibroblasts to hepatic lineages could offer a solution to this problem but so far has only been achieved with mouse cells. here, we generated human induced hepatocytes (hiheps) from fibroblasts by lentiviral expression of foxa3, hnf1a, and hnf4a. hiheps express hepatic gene programs, can be expanded in vitro, and display functions characteristic of mature hepatocytes, including cytochrome p450 enzyme activity and biliary drug clearance. upon transplantation into mice with concanavalin-a-induced acute liver failure and fatal metabolic liver disease due to fumarylacetoacetate dehydrolase (fah) deficiency, hiheps restore the liver function and prolong survival. collectively, our results demonstrate successful lineage conversion of nonhepatic human cells into mature hepatocytes with potential for biomedical and pharmaceutical applications. |
| 学科主题 | 物理化学 |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | cell & tissue engineering ; cell biology |
| 研究领域[WOS] | cell biology |
| 关键词[WOS] | bioartificial liver ; drug-metabolism ; transcription factors ; p53-deficient mice ; in-vivo ; cells ; conversion ; challenges ; induction ; failure |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000332533400013 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/144005]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, Shanghai 200031, Peoples R China 2.Second Mil Med Univ, Dept Cell Biol, Shanghai 200433, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Safety Evaluat & Res, Shanghai 201203, Peoples R China 4.Shanghai Jiao Tong Univ, Sch Med, Int Peace Matern & Child Hlth Hosp, Shanghai 200030, Peoples R China 5.Shanghai Jiao Tong Univ, Sch Med, Renji Hosp, Shanghai 200127, Peoples R China 6.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China 7.Inner Mongolia Univ, Key Lab Natl Educ, Minist Mammalian Reprod Biol & Biotechnol, Hohhot 010021, Peoples R China 8.Univ Minnesota, Stem Cell Inst, Dept Lab Med & Pathol, Minneapolis, MN 55455 USA 9.Hepatoscience Inc, Palo Alto, CA 94303 USA |
| 推荐引用方式 GB/T 7714 | Huang, Pengyu,Zhang, Ludi,Gao, Yimeng,et al. Direct Reprogramming of Human Fibroblasts to Functional and Expandable Hepatocytes[J]. cell stem cell,2014,14(3):370-384. |
| APA | Huang, Pengyu.,Zhang, Ludi.,Gao, Yimeng.,He, Zhiying.,Yao, Dan.,...&Hui, Lijian.(2014).Direct Reprogramming of Human Fibroblasts to Functional and Expandable Hepatocytes.cell stem cell,14(3),370-384. |
| MLA | Huang, Pengyu,et al."Direct Reprogramming of Human Fibroblasts to Functional and Expandable Hepatocytes".cell stem cell 14.3(2014):370-384. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。