A metabolomics-based method for studying the effect of yfcC gene in Escherichia coli on metabolism
文献类型：期刊论文
| 作者 | Wang, Xiyue1; Xie, Yuping2; Gao, Peng1; Zhang, Sufang3; Tan, Haidong3; Yang, Fengxu2; Lian, Rongwei2; Tian, Jing2; Xu, Guowang1 |
| 刊名 | analytical biochemistry
 |
| 出版日期 | 2014-04-15 |
| 卷号 | 451期号:1页码:48-55 |
| 关键词 | Metabolomics Gas chromatography-mass spectrometry Escherichia coli yfcC Metabolic profiling |
| 通讯作者 | 高鹏 ; 田晶 |
| 英文摘要 | metabolomics is a potent tool to assist in identifying the function of unknown genes through analysis of metabolite changes in the context of varied genetic backgrounds. however, the availability of a universal unbiased profiling analysis is still a big challenge. in this study, we report an optimized metabolic profiling method based on gas chromatography-mass spectrometry for escherichia coli. it was found that physiological saline at -80 degrees c could ensure satisfied metabolic quenching with less metabolite leakage. a solution of methanol/water (21:79, v/v) was proved to be efficient for intracellular metabolite extraction. this method was applied to investigate the metabolome difference among wild-type e. coli, its yfcc deletion, and overexpression mutants. statistical and bioinformatic analysis of the metabolic profiling data indicated that the expression of yfcc potentially affected the metabolism of glyoxylate shunt. this finding was further validated by real-time quantitative polymerase chain reactions showing that expression of acea and aceb, the key genes in glyoxylate shunt, was upregulated by yfcc. this study exemplifies the robustness of the proposed metabolic profiling analysis strategy and its potential roles in investigating unknown gene functions in view of metabolome difference. (c) 2014 elsevier inc. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | biochemical research methods ; biochemistry & molecular biology ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | functional genomics tool ; mass-spectrometry ; microbial metabolomics ; optimization ; bacteria ; impact ; k-12 ; reconstruction ; metaboanalyst ; annotation |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000334144200011 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/144013]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Dalian Polytech Univ, Sch Bioengn, Dalian 116034, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiyue,Xie, Yuping,Gao, Peng,et al. A metabolomics-based method for studying the effect of yfcC gene in Escherichia coli on metabolism[J]. analytical biochemistry,2014,451(1):48-55. |
| APA | Wang, Xiyue.,Xie, Yuping.,Gao, Peng.,Zhang, Sufang.,Tan, Haidong.,...&Xu, Guowang.(2014).A metabolomics-based method for studying the effect of yfcC gene in Escherichia coli on metabolism.analytical biochemistry,451(1),48-55. |
| MLA | Wang, Xiyue,et al."A metabolomics-based method for studying the effect of yfcC gene in Escherichia coli on metabolism".analytical biochemistry 451.1(2014):48-55. |
入库方式： OAI收割
来源：大连化学物理研究所
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