Identification of UshA as a major enzyme for NAD degradation in Escherichia coli
文献类型：期刊论文
| 作者 | Wang, Lei1,4; Zhou, Yongjin J.1; Ji, Debin1; Lin, Xinping1,4; Liu, Yuxue1,4; Zhang, Yixin1,4; Liu, Wujun1; Zhao, Zongbao K.1,2,3 |
| 刊名 | enzyme and microbial technology
 |
| 出版日期 | 2014-05-10 |
| 卷号 | 58-59期号:1页码:75-79 |
| 关键词 | Whole-cell biocatalysis Nicotinamide adenine dinucleotide NAD degradation UshA Pyrophosphatase 5 '-Nucleotidase |
| 通讯作者 | 赵宗保 |
| 英文摘要 | nicotinamide adenine dinucleotide (nad) and its reduced form nadh are essential cofactors for many redox biocatalysts. because these cofactors are consumed in stoichiometric amounts, whole-cell biocatalysts have been routinely employed in order to reduce the costs. to further improve the efficacy of redox biocatalysts, it is essential to maintain the stability of nicotinamide cofactors, for which it is attractive to block degradation pathways for nad(h). while the biosynthesis of nad(h) has been well studied, it is less understood how nad(h) are degraded. here we demonstrated that usha was a major periplasmic enzyme for nad degradation in escherichia colt. purified recombinant usha showed high pyrophosphatase activity with the catalytic efficiencies for hydrolysis of nad and nadh at 3.7 mu m-1 s(-1) and 1.4 mu m-1 s(-1), respectively. deletion of the usha gene from the chromosome led to faster cell growth and improved extracellular nad stability by 3-fold under conditions similar to whole-cell biocatalysis. these results significantly enriched our understanding on nad metabolism, and should facilitate many applications including designing more robust redox biocatalysts. (c) 2014 elsevier inc. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 学科主题 | 物理化学 |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | hydrolase ; cloning ; redistribution ; availability ; gene |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000336464600011 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/144050]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian Natl Lab Clean Energy, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Lei,Zhou, Yongjin J.,Ji, Debin,et al. Identification of UshA as a major enzyme for NAD degradation in Escherichia coli[J]. enzyme and microbial technology,2014,58-59(1):75-79. |
| APA | Wang, Lei.,Zhou, Yongjin J..,Ji, Debin.,Lin, Xinping.,Liu, Yuxue.,...&Zhao, Zongbao K..(2014).Identification of UshA as a major enzyme for NAD degradation in Escherichia coli.enzyme and microbial technology,58-59(1),75-79. |
| MLA | Wang, Lei,et al."Identification of UshA as a major enzyme for NAD degradation in Escherichia coli".enzyme and microbial technology 58-59.1(2014):75-79. |
入库方式： OAI收割
来源：大连化学物理研究所
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