Functional integration of multiple genes into the genome of the oleaginous yeast Rhodosporidium toruloides
文献类型：期刊论文
| 作者 | Lin, Xinping1,2; Wang, Yanan1,2; Zhang, Sufang1,3; Zhu, Zhiwei1,2; Zhou, Yongjin J.1; Yang, Fan4; Sun, Wenyi1; Wang, Xueying1; Zhao, Zongbao K.1,3 |
| 刊名 | fems yeast research
 |
| 出版日期 | 2014-06-01 |
| 卷号 | 14期号:4页码:547-555 |
| 关键词 | Rhodosporidium toruloides Agrobacterium-mediated transformation gene integration antibiotic resistance |
| 通讯作者 | 赵宗保 |
| 英文摘要 | the basidiomycetous yeast rhodosporidium toruloides represents an excellent producer for microbial lipids and carotenoids. however, further rational engineering of this unconventional yeast remains challenging partially because of the absence of efficient and reliable transformation method. in this study, we developed an agrobacterium-mediated transformation (atmt) protocol for effective gene integration into the r.toruloides genome. both haploid and diploid strains were successfully modified, and the integration was confirmed by colony pcr, western blot analysis and genome walking. we further demonstrated that multiple genes could be integrated by consecutive atmt, leading to engineered strains simultaneously resistant to multiple antibiotics. our results provided a practical method for functional integration and expression of exogenous genes in r.toruloides, which should facilitate the development of genetic tools and the construction of superior strains to produce biofuel molecules and biochemicals. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 学科主题 | 物理化学 |
| 类目[WOS] | biotechnology & applied microbiology ; microbiology ; mycology |
| 研究领域[WOS] | biotechnology & applied microbiology ; microbiology ; mycology |
| 关键词[WOS] | cryptococcus-neoformans ; lipid production ; homologous recombination ; insertional mutagenesis ; microbial-production ; ustilago-maydis ; red yeasts ; transformation ; agrobacterium ; fungi |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000337584500004 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/144055]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Dalian Natl Lab Clean Energy, Dalian, Peoples R China 4.Dalian Polytech Univ, Sch Biol Engn, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, Xinping,Wang, Yanan,Zhang, Sufang,et al. Functional integration of multiple genes into the genome of the oleaginous yeast Rhodosporidium toruloides[J]. fems yeast research,2014,14(4):547-555. |
| APA | Lin, Xinping.,Wang, Yanan.,Zhang, Sufang.,Zhu, Zhiwei.,Zhou, Yongjin J..,...&Zhao, Zongbao K..(2014).Functional integration of multiple genes into the genome of the oleaginous yeast Rhodosporidium toruloides.fems yeast research,14(4),547-555. |
| MLA | Lin, Xinping,et al."Functional integration of multiple genes into the genome of the oleaginous yeast Rhodosporidium toruloides".fems yeast research 14.4(2014):547-555. |
入库方式： OAI收割
来源：大连化学物理研究所
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