Preparation of Monolithic Polymer Columns with Homogeneous Structure via Photoinitiated Thiol-yne Click Polymerization and Their Application in Separation of Small Molecules
文献类型：期刊论文
| 作者 | Liu, Zhongshan1,2; Ou, Junjie1; Lin, Hui1,2; Wang, Hongwei1; Liu, Zheyi1,2; Dong, Jing1; Zou, Hanfa1 |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2014-12-16 |
| 卷号 | 86期号:24页码:12334-12340 |
| 通讯作者 | 欧俊杰 ; 邹汉法 |
| 英文摘要 | two monolithic polymer columns were directly prepared in the uv-transparent fused-silica capillaries via photoinitiated thiol-yne click polymerization of 1,7-octadiyne (ody) with a dithiol (1,6-hexanedithiol, 2sh) or a tetrathiol (pentaerythriol tetrakis(3-mercaptopropionate), 4sh) within 15 min. the rapid polymerization provided a time-saving approach to optimize preparation conditions. then, two porogenic systems of diethylene glycol diethyl ether (degde)/tetrahydrofuran (thf) and degde/poly(ethylene glycol) (peg, mn = 200) were found to effectively control the porous structure of two kinds of polymeric monoliths (o2sh and o4sh), respectively. the almost disappearance of thiol and alkynyl vibrations (2560 and 2115 cm(-1), respectively) in infrared spectra and raman spectra indicated a high conversion of the thiol-yne polymerization reaction. the thiol-yne polymerization was further proved by analyzing the energy-dispersive x-ray spectrum (eds), maldi-tof mass spectrum, and elemental data. scanning electron microscopy (sem) images showed the monolithic polymer columns with homogeneous porous structure and macropore size of 0.5-1.0 mu m, which facilitated the minimum plate heights of 10.0-12.0 mum for alkylbenzenes in reversed-phase liquid chromatography (rplc). the low values of the a and c terms (<1.0 mu m and <15.5 ms, respectively) in the van deemter equation were similar to those obtained by some monolithic silica columns. the bsa tryptic digest was also separated on the monolithic polymer column by clc-ms/ms. the result with 85% protein coverage was better than those given by some hybrid monolithic columns. the monolithic polymer columns were further applied for separation of phenols, natural products, and standard proteins and demonstrated satisfactory separation ability. |
| 学科主题 | 物理化学 |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | performance liquid-chromatography ; polyhedral oligomeric silsesquioxane ; ring-opening polymerization ; capillary electrochromatography ; stationary phases ; ene chemistry ; silica ; morphology ; efficient ; photopolymerization |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000346683900061 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/144257]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Zhongshan,Ou, Junjie,Lin, Hui,et al. Preparation of Monolithic Polymer Columns with Homogeneous Structure via Photoinitiated Thiol-yne Click Polymerization and Their Application in Separation of Small Molecules[J]. analytical chemistry,2014,86(24):12334-12340. |
| APA | Liu, Zhongshan.,Ou, Junjie.,Lin, Hui.,Wang, Hongwei.,Liu, Zheyi.,...&Zou, Hanfa.(2014).Preparation of Monolithic Polymer Columns with Homogeneous Structure via Photoinitiated Thiol-yne Click Polymerization and Their Application in Separation of Small Molecules.analytical chemistry,86(24),12334-12340. |
| MLA | Liu, Zhongshan,et al."Preparation of Monolithic Polymer Columns with Homogeneous Structure via Photoinitiated Thiol-yne Click Polymerization and Their Application in Separation of Small Molecules".analytical chemistry 86.24(2014):12334-12340. |
入库方式： OAI收割
来源：大连化学物理研究所
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