Proteomic analysis of protein methylation in the yeast Saccharomyces cerevisiae
文献类型：期刊论文
| 作者 | Wang, Keyun1,2; Zhou, Yongjin J.1; Liu, Hongwei1; Cheng, Kai1,2; Mao, Jiawei1,2; Wang, Fangjun1; Liu, Wujun1; Ye, Mingliang1; Zhao, Zongbao K.1; Zou, Hanfa1 |
| 刊名 | journal of proteomics
 |
| 出版日期 | 2015-01-30 |
| 卷号 | 114期号:1页码:226-233 |
| 关键词 | Saccharomyces cerevisiae Methylation Proteomics LC/MS/MS |
| 通讯作者 | 赵宗保 ; 邹汉法 |
| 英文摘要 | protein methylation catalyzed by sam-dependent methyltransferase represents a major ptm involved in many important biological processes. because methylation can occur on nitrogen, oxygen and sulfur centers and multiple methylation states exist on the nitrogen centers, methylproteome remains poorly documented. here we present the methylation by isotope labeled sam (mils) strategy for a highly-confident analysis of the methylproteome of the yeast saccharomyces cerevisiae based on the online multidimensional mu hplc/ms/ms technology. we identified 43 methylated proteins, containing 68 methylation events associated with 64 methylation sites. more than 90% of these methylation events were previously unannotated in uniprot database. our results indicated, 1) over 2.6% of identified s. cerevisiae proteins are methylated, 2) the amino acid residue preference of protein methylation follows the order lys arg >> asp > asn approximate to gln approximate to his > glu > cys, and 3) the methylation state on nitrogen center is largely exclusive. as our dataset covers various types of methylation centers, it provides rich information about yeast methylproteome and should significantly contribute to the field of protein methylaidon. |
| 学科主题 | 物理化学 |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemical research methods |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | arginine methylation ; lysine-methylation ; mass-spectrometry ; posttranslational modifications ; s-adenosylmethionine ; in-vivo ; identification ; methionine ; scale ; translation |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000348949100018 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/144284]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Keyun,Zhou, Yongjin J.,Liu, Hongwei,et al. Proteomic analysis of protein methylation in the yeast Saccharomyces cerevisiae[J]. journal of proteomics,2015,114(1):226-233. |
| APA | Wang, Keyun.,Zhou, Yongjin J..,Liu, Hongwei.,Cheng, Kai.,Mao, Jiawei.,...&Zou, Hanfa.(2015).Proteomic analysis of protein methylation in the yeast Saccharomyces cerevisiae.journal of proteomics,114(1),226-233. |
| MLA | Wang, Keyun,et al."Proteomic analysis of protein methylation in the yeast Saccharomyces cerevisiae".journal of proteomics 114.1(2015):226-233. |
入库方式： OAI收割
来源：大连化学物理研究所
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