Enrichment and separation techniques for large-scale proteomics analysis of the protein post-translational modifications
文献类型：期刊论文
| 作者 | Huang, Junfeng1,2; Wang, Fangjun1; Ye, Mingliang1; Zou, Hanfa1 |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2014-12-12 |
| 卷号 | 1372期号:1页码:1-17 |
| 关键词 | Post-translational modifications Enrichment Multi-dimensional liquid chromatography separation Mass spectrometry Protein phosphorylation Protein glycosylation |
| 通讯作者 | 叶明亮 ; 邹汉法 |
| 英文摘要 | comprehensive analysis of the post-translational modifications (ptms) on proteins at proteome level is crucial to elucidate the regulatory mechanisms of various biological processes. in the past decades, thanks to the development of specific ptm enrichment techniques and efficient multidimensional liquid chromatography (lc) separation strategy, the identification of protein ptms have made tremendous progress. a huge number of modification sites for some major protein ptms have been identified by proteomics analysis. in this review, we first introduced the recent progresses of ptm enrichment methods for the analysis of several major ptms including phosphorylation, glycosylation, ubiquitination, acetylation, methylation, and oxidation/reduction status. we then briefly summarized the challenges for ptm enrichment. finally, we introduced the fractionation and separation techniques for efficient separation of ptm peptides in large-scale ptm analysis. (c) 2014 elsevier b.v. all rights reserved. |
| 学科主题 | 物理化学 |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | hydrophilic interaction chromatography ; ion affinity-chromatography ; tandem mass-spectrometry ; solid-phase extraction ; n-linked glycopeptides ; multidimensional liquid-chromatography ; monolithic capillary column ; highly selective enrichment ; glutathionylated cellular-proteins ; multiply phosphorylated peptides |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000347020900001 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/144392]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China 2.Univ Chinese Acad Sci, Being, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Junfeng,Wang, Fangjun,Ye, Mingliang,et al. Enrichment and separation techniques for large-scale proteomics analysis of the protein post-translational modifications[J]. journal of chromatography a,2014,1372(1):1-17. |
| APA | Huang, Junfeng,Wang, Fangjun,Ye, Mingliang,&Zou, Hanfa.(2014).Enrichment and separation techniques for large-scale proteomics analysis of the protein post-translational modifications.journal of chromatography a,1372(1),1-17. |
| MLA | Huang, Junfeng,et al."Enrichment and separation techniques for large-scale proteomics analysis of the protein post-translational modifications".journal of chromatography a 1372.1(2014):1-17. |
入库方式： OAI收割
来源：大连化学物理研究所
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