Boronate Affinity Monolith with a Gold Nanoparticle-Modified Hydrophilic Polymer as a Matrix for the Highly Specific Capture of Glycoproteins
文献类型:期刊论文
作者 | Wu, Ci1,2; Liang, Yu1; Zhao, Qun1,2; Qu, Yanyan1,2; Zhang, Shen1,2; Wu, Qi1,2; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1 |
刊名 | chemistry-a european journal |
出版日期 | 2014-07-07 |
卷号 | 20期号:28页码:8737-8743 |
关键词 | boron glycoproteins gold nanoparticles polymers |
通讯作者 | 张丽华 |
英文摘要 | as low abundance is the great obstacle for glycoprotein analysis, the development of materials with high efficiency and selectivity for glycoprotein enrichment is a prerequisite in glycoproteome research. herein, we report a new kind of hydrophilic boronate affinity monolith by attaching 4-mercaptophenylboronic acid (mpba) with 2-mercaptoethylamine (mpa) on the gold nanoparticle-modified poly(glycidyl methacrylate-co-poly(ethylene glycol) diacrylate)) monolith for glycoprotein enrichment. with poly(ethylene glycol) diacrylate as the cross-linker and the further modification of gold nanoparticles, the matrix has advantages of good hydrophilicity and enhanced surface area, which are beneficial to improve the enrichment selectivity and efficiency for glycoproteins. the attachment of mpba and mpa provide intramolecular b-n coordination, which could further enhance the specificity of glycoprotein capture. such a boronate affinity monolith was applied to enrich horseradish peroxidase (hrp) from the mixture of hrp and bovine serum albumin (bsa), and high selectivity was obtained even at a mass ratio of 1:1000. in addition, the binding capacity of ovalbumin on such monolith reached 390 mu g g(-1). furthermore, the average recovery of hrp on the prepared affinity monoliths was (84.8 +/- 1.9)%, obtained in three times enrichment with the same column. finally, the boronate affinity monolith was successfully applied for the human-plasma glycoproteome analysis. as a result, 160 glycoproteins were credibly identified from 9 mu g of human plasma, demonstrating the great potential of such a monolith for large-scale glycoproteome research. |
学科主题 | 物理化学 |
WOS标题词 | science & technology ; physical sciences |
类目[WOS] | chemistry, multidisciplinary |
研究领域[WOS] | chemistry |
关键词[WOS] | cis-diol ; liquid-chromatography ; selective enrichment ; hydrazide chemistry ; mass-spectrometry ; body-fluids ; column ; identification ; glycosylation ; capillary |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000338768900033 |
公开日期 | 2016-05-09 |
源URL | [http://cas-ir.dicp.ac.cn/handle/321008/144394] |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
作者单位 | 1.Chinese Acad Sci, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem Dalian Chem Phys, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
推荐引用方式 GB/T 7714 | Wu, Ci,Liang, Yu,Zhao, Qun,et al. Boronate Affinity Monolith with a Gold Nanoparticle-Modified Hydrophilic Polymer as a Matrix for the Highly Specific Capture of Glycoproteins[J]. chemistry-a european journal,2014,20(28):8737-8743. |
APA | Wu, Ci.,Liang, Yu.,Zhao, Qun.,Qu, Yanyan.,Zhang, Shen.,...&Zhang, Yukui.(2014).Boronate Affinity Monolith with a Gold Nanoparticle-Modified Hydrophilic Polymer as a Matrix for the Highly Specific Capture of Glycoproteins.chemistry-a european journal,20(28),8737-8743. |
MLA | Wu, Ci,et al."Boronate Affinity Monolith with a Gold Nanoparticle-Modified Hydrophilic Polymer as a Matrix for the Highly Specific Capture of Glycoproteins".chemistry-a european journal 20.28(2014):8737-8743. |
入库方式: OAI收割
来源:大连化学物理研究所
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