Guanidinium functionalized superparamagnetic silica spheres for selective enrichment of phosphopeptides and intact phosphoproteins from complex mixtures
文献类型：期刊论文
| 作者 | Deng, Qiliang1,2; Wu, Jianhua2; Chen, Yang1; Zhang, Zhijun2; Wang, Yang2; Fang, Guozhen2; Wang, Shuo2; Zhang, Yukui3 |
| 刊名 | journal of materials chemistry b
 |
| 出版日期 | 2014-02-28 |
| 卷号 | 2期号:8页码:1048-1058 |
| 通讯作者 | shuowang |
| 英文摘要 | phosphorylation of protein regulates nearly all biological processes in nature. the development of enrichment techniques for phosphorylated proteins is vital to systematic identification and characterization of phosphoproteins. here, a general strategy for highly efficient capture of intact phosphorylated proteins from protein mixtures has been developed by using guanidine functionalized superparamagnetic microspheres (denoted as fe3o4@sio2@gdn). the fe3o4@sio2@gdn was prepared by modifying fe3o4@sio2 with 3-guanidopropyl triethoxysilane as a functionalization monomer. the resulting materials could specifically and selectively recognize phosphoproteins and showed high binding capacities for model phosphoproteins (78.8 mg g(-1) for ovalbumin (ova) and 59.6 mg g(-1) for beta-casein (beta-cas), respectively). the feasibility of the resulting material for phosphoprotein enrichment has also been demonstrated by selectively binding and capturing phosphoproteins from complex protein mixtures and real samples (milk, egg, and tissue protein extract from a mouse liver), respectively. in addition, the selective enrichment of phosphopeptides has also been investigated. the proposed technique showed application potential for phosphoprotein and phosphopeptide enrichment. |
| WOS标题词 | science & technology ; technology |
| 学科主题 | 物理化学 |
| 类目[WOS] | materials science, biomaterials |
| 研究领域[WOS] | materials science |
| 关键词[WOS] | metal-affinity-chromatography ; phosphorylated proteins ; mass-spectrometry ; recognition ; purification ; strategies ; proteomics ; receptors ; mechanism |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000331500800012 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/144437]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Tianjin Univ Sci & Technol, Dept Sci, Tianjin 300457, Peoples R China 2.Tianjin Univ Sci & Technol, Key Lab Food Nutr & Safety, Minist Educ China, Tianjin 300457, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Deng, Qiliang,Wu, Jianhua,Chen, Yang,et al. Guanidinium functionalized superparamagnetic silica spheres for selective enrichment of phosphopeptides and intact phosphoproteins from complex mixtures[J]. journal of materials chemistry b,2014,2(8):1048-1058. |
| APA | Deng, Qiliang.,Wu, Jianhua.,Chen, Yang.,Zhang, Zhijun.,Wang, Yang.,...&Zhang, Yukui.(2014).Guanidinium functionalized superparamagnetic silica spheres for selective enrichment of phosphopeptides and intact phosphoproteins from complex mixtures.journal of materials chemistry b,2(8),1048-1058. |
| MLA | Deng, Qiliang,et al."Guanidinium functionalized superparamagnetic silica spheres for selective enrichment of phosphopeptides and intact phosphoproteins from complex mixtures".journal of materials chemistry b 2.8(2014):1048-1058. |
入库方式： OAI收割
来源：大连化学物理研究所
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