Label-free quantification of differentially expressed proteins in mouse liver cancer cells with high and low metastasis rates by a SWATH acquisition method
文献类型：期刊论文
| 作者 | Yan ZiQi; Zhou Yuan; Shan YiChu; Wu Qi; Zhang Shen; Liang Zhen; Zhang LiHua; Zhang YuKui |
| 刊名 | science china-chemistry
 |
| 出版日期 | 2014-05-01 |
| 卷号 | 57期号:5页码:718-722 |
| 关键词 | label-free quantification SWATH acquisition differentially expressed protein liver cancer metastasis rate |
| 通讯作者 | 张丽华 |
| 英文摘要 | label-free quantification is a valuable tool for the analysis of differentially expressed proteins identified by mass spectrometry methods. herein, we used a new strategy: data-dependent acquisition mode identification combined with label-free quantification by swath acquisition mode, to study the differentially expressed proteins in mouse liver cancer metastasis cells. a total of 1528 protein groups were identified, among which 1159 protein groups were quantified and 249 protein groups were observed as differentially expressed proteins (86 proteins up-regulated and 163 down-regulated). this method provides a commendable solution for the identification and quantification of differentially expressed proteins in biological samples. |
| 学科主题 | 物理化学 |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | tandem mass-spectrometry ; lymphatic metastasis ; shotgun proteomics ; plasma-proteins ; biomarker ; identification ; carcinoma ; spectra ; lines ; ms |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000335735200009 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/144438]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan ZiQi,Zhou Yuan,Shan YiChu,et al. Label-free quantification of differentially expressed proteins in mouse liver cancer cells with high and low metastasis rates by a SWATH acquisition method[J]. science china-chemistry,2014,57(5):718-722. |
| APA | Yan ZiQi.,Zhou Yuan.,Shan YiChu.,Wu Qi.,Zhang Shen.,...&Zhang YuKui.(2014).Label-free quantification of differentially expressed proteins in mouse liver cancer cells with high and low metastasis rates by a SWATH acquisition method.science china-chemistry,57(5),718-722. |
| MLA | Yan ZiQi,et al."Label-free quantification of differentially expressed proteins in mouse liver cancer cells with high and low metastasis rates by a SWATH acquisition method".science china-chemistry 57.5(2014):718-722. |
入库方式： OAI收割
来源：大连化学物理研究所
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