1-Dodecyl-3-Methylimidazolium Chloride-Assisted Sample Preparation Method for Efficient Integral Membrane Proteome Analysis
文献类型：期刊论文
| 作者 | Zhao, Qun1,2; Fang, Fei1,2; Liang, Yu1; Yuan, Huiming1; Yang, Kaiguang1; Wu, Qi1,2; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1 |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2014-08-05 |
| 卷号 | 86期号:15页码:7544-7550 |
| 通讯作者 | 张丽华 |
| 英文摘要 | due to their extremely hydrophobic nature, the analysis of integral membrane proteins (imps) is of great challenge. although various additives have been applied to improve the solubility of imps, they still suffer from low solubilization efficiency, incompatibility with trypsin digestion, or interference with ms detection. herein, the systematic study on the effect of ionic liquid structure on membrane protein solubilization and trypsin biocompatibility was performed, based on which 1-dodecyl-3-methylimidazolium chloride (c12im-cl) was selected for the sample preparation of imps. compared with other commonly used additives, such as sodium dodecyl sulfate (sds), rapigest, and methanol, c12im-cl showed the best performance. in addition, with a strong cation exchange trap column, it could be easily removed after trypsin digestion, which not only was beneficial to avoid protein precipitation during digestion but also had no adverse effect on lc-ms-based separation and detection. such a c12im-cl-assisted sample preparation method was further applied to the membrane proteome analysis of rat brain. compared with the sds-assisted method, 1.4 and 3.5 times improvement on the identified imp and hydrophobic peptide number were achieved (251 vs 178, and 982 vs 279). all these results demonstrated that the c12im-cl-assisted sample preparation method is of great promise to promote the large-scale membrane proteome profiling. |
| 学科主题 | 物理化学 |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | tandem mass-spectrometry ; ionic liquids ; tryptic digestion ; infrared-spectroscopy ; lc-ms/ms ; proteins ; solubilization ; methanol ; identification ; chromatography |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000340081100057 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/144459]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Qun,Fang, Fei,Liang, Yu,et al. 1-Dodecyl-3-Methylimidazolium Chloride-Assisted Sample Preparation Method for Efficient Integral Membrane Proteome Analysis[J]. analytical chemistry,2014,86(15):7544-7550. |
| APA | Zhao, Qun.,Fang, Fei.,Liang, Yu.,Yuan, Huiming.,Yang, Kaiguang.,...&Zhang, Yukui.(2014).1-Dodecyl-3-Methylimidazolium Chloride-Assisted Sample Preparation Method for Efficient Integral Membrane Proteome Analysis.analytical chemistry,86(15),7544-7550. |
| MLA | Zhao, Qun,et al."1-Dodecyl-3-Methylimidazolium Chloride-Assisted Sample Preparation Method for Efficient Integral Membrane Proteome Analysis".analytical chemistry 86.15(2014):7544-7550. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。