Metabolomics Study of Hepatocellular Carcinoma: Discovery and Validation of Serum Potential Biomarkers by Using Capillary Electrophoresis-Mass Spectrometry
文献类型：期刊论文
| 作者 | Zeng, Jun1; Yin, Peiyuan1; Tan, Yexiong2; Dong, Liwei2; Hu, Chunxiu1; Huang, Qiang1; Lu, Xin1; Wang, Hongyang2; Xu, Guowang1 |
| 刊名 | journal of proteome research
 |
| 出版日期 | 2014-07-01 |
| 卷号 | 13期号:7页码:3420-3431 |
| 关键词 | hepatocellular carcinoma HCC metabolomics serum biomarkers capillary electrophoresis mass spectrometry |
| 通讯作者 | hongyangwang ; 许国旺 |
| 英文摘要 | hepatocellular carcinoma (hcc) is one of the most lethal malignancies. the lack of effective screening methods for early diagnosis has been a longstanding bottleneck to improve the survival rate. in the present study, a capillary electrophoresis-time-of-flight mass spectrometry (ce-tof/ms)-based metabolomics method was employed to discover novel biomarkers for hcc. a total of 183 human serum specimens (77 sera in discovery set and 106 sera in external validation set) were enrolled in this study, and a "serum biomarker model" including tryptophan, glutamine, and 2-hydroxybutyric acid was finally established based on the comprehensive screening and validation workflow. this model was evaluated as an effective tool in that area under the receiver operating characteristic curve reached 0.969 in the discovery set and 0.99 in the validation set for diagnosing hcc from non-hcc (health and cirrhosis). furthermore, this model enabled the discrimination of small hcc from precancer cirrhosis with an auc of 0.976, highlighting the potential of early diagnosis. the biomarker model is effective for those a-fetoprotein (afp) false-negative and false-postive subjects, indicating the complementary function to conventional tumor marker afp. this study demonstrates the promising potential of ce-ms-based metabolomics approach in finding biomarkers for disease diagnosis and providing special insights into tumor metabolism. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 学科主题 | 物理化学 |
| 类目[WOS] | biochemical research methods |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | indoleamine 2,3-dioxygenase ; arginine deprivation ; glutamine-metabolism ; tumor-cells ; cancer ; depletion ; growth ; proliferation ; inhibition ; diagnosis |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000338693400026 |
| 公开日期 | 2016-05-09 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/144483]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Second Mil Med Univ, Int Cooperat Lab Signal Transduct, Eastern Hepatobiliary Surg Inst, Shanghai 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zeng, Jun,Yin, Peiyuan,Tan, Yexiong,et al. Metabolomics Study of Hepatocellular Carcinoma: Discovery and Validation of Serum Potential Biomarkers by Using Capillary Electrophoresis-Mass Spectrometry[J]. journal of proteome research,2014,13(7):3420-3431. |
| APA | Zeng, Jun.,Yin, Peiyuan.,Tan, Yexiong.,Dong, Liwei.,Hu, Chunxiu.,...&Xu, Guowang.(2014).Metabolomics Study of Hepatocellular Carcinoma: Discovery and Validation of Serum Potential Biomarkers by Using Capillary Electrophoresis-Mass Spectrometry.journal of proteome research,13(7),3420-3431. |
| MLA | Zeng, Jun,et al."Metabolomics Study of Hepatocellular Carcinoma: Discovery and Validation of Serum Potential Biomarkers by Using Capillary Electrophoresis-Mass Spectrometry".journal of proteome research 13.7(2014):3420-3431. |
入库方式： OAI收割
来源：大连化学物理研究所
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